Core 2 Immune Monitoring

摘要 RadCCORE的免疫监测核心(核心2，以前的核心C)自#年首次获奖以来一直存在 2005年。核心由技术和服务组成，通过位于 杜克人类疫苗研究所(DHVI)和NIAID建造的杜克地区生物遏制实验室 (RBL)：流式细胞术和细胞分选设施，以及DHVI/RBL免疫股。RadCcore 免疫监测核心公司将定期为调查人员提供高质量、最先进的细胞分类和 表型，多重细胞因子谱阵列，和T细胞谱系监测。本网站提供的服务 续订反映了第1-14年中的实际核心利用率，并已更新以满足新的和 更新计划中的创新项目。RadCCORE免疫的特定目标(服务任务) 监测核心是：1)提供最先进的、多色、荧光激活的细胞分类和 免疫表型支持；2)为生物样本提供有针对性的多重生物标志物分析，例如 组织培养上清液、血清/血浆和肺灌洗液，采用Luminex珠基分析；3) 通过执行T细胞受体切除环量化和T细胞免疫重建监测 外周T细胞受体β可变基因利用的T细胞谱系深度测序。通过在 与所有四个RadCCORE项目合作，我们将优化分析以满足他们的需求和更多 有效利用RadCCORE免疫监测系统提供的最先进的仪器 核心。对我们所有三个目标至关重要的是对仪器和分析的持续质量控制的承诺， 以及对预测和满足RadCCORE调查人员未来需求的坚定承诺。

Abstract The Immune Monitoring Core (Core 2, previously Core C) for RadCCORE has existed since the initial award in 2005. The Core is comprised of technologies and services offered through two shared resource facilities in the Duke Human Vaccine Institute (DHVI) and the NIAID-constructed Duke Regional Biocontainment Laboratory (RBL): the Flow Cytometry and Cell Sorting facility, and the DHVI/RBL Immunology Unit. The RadCCORE Immune Monitoring Core will routinely provide investigators with high-quality, state-of-the-art cell sorting and phenotyping, multiplex cytokine profiling arrays, and T cell repertoire monitoring. Services offered in this renewal reflect actual Core utilization in years 1-14 and have been updated to meet the needs of the new and innovative projects in the renewal program. Specific AIms (Service Tasks) of the RadCCORE Immune Monitoring Core are: 1) Provide state-of-the-art, polychromatic, fluorescence activated cell sorting and immunophenotyping support; 2) Provide targeted multiplex biomarker profiling of biological samples, such as tissue culture supernatant, serum/plasma, and lung lavage fluid, using Luminex bead-based assays; and 3) Provide T cell immune reconstitution monitoring by performing T cell receptor excision circle quantification and T cell repertoire deep-sequencing of peripheral T cell receptor beta variable gene utilization. By working in collaboration with all four RadCCORE projects we will optimize assays to meet their needs and more effectively utilize the state-of-the-art instrumentation available through the RadCCORE Immune Monitoring Core. Critical to all three of our aims is a commitment to ongoing quality control of instruments and assays, and a strong commitment to anticipate and meet the future needs of the RadCCORE investigators.