Digital Spatial Profiler

摘要 要求为NanoStringGeoMxTM数字空间剖面仪(数字空间剖面仪)提供资金。这台仪器是一个多路复用器 便于空间基因表达分析的成像设备。这一工具将提供独特的附加值 致纽约西部罗斯威尔公园综合癌症中心及周边地区的调查人员 约克市。DSP仪器将福尔马林固定组织中的空间信息与 下游分子分析。免疫组织化学(IHC)等传统技术或 免疫荧光(IF)提供空间信息，但受到荧光团的限制。相比之下，单个细胞 测序提供了关于单个细胞的深层信息，但空间信息丢失了。该数字信号处理器能够准确地 并灵敏地检测多达1800个RNA分子和96个蛋白质，空间分辨率使用 RNA探针和抗体。这种形式的数据在癌症研究的背景下特别相关(例如 肿瘤异质性、肿瘤微环境)以及其他生物学学科(如发育 生物学、神经科学)。虽然罗斯威尔公园有用于空间分析和基因组学的工具，但没有 可与方圆200英里的数字信号处理器相媲美的技术。仪器将被放置在中心用于 专注于高级组织分析并与基因组学并列的个性化医学(CPM) Roswell Park的共享资源(GSR)，专注于分子分析。此结构提供了 独特的病理和基因组专业知识的结合，以适当地利用DSP仪器。那里 该仪器的8个主要用户加在一起将使用约80%的仪器容量。剩余 仪器容量将通过我们的在线调度和 预订系统。CPM/GSR董事将制定一份业务计划，并成立一个多学科咨询委员会 负责文书监督，同时该机构将通过服务合同为文书提供支持， 人事，以及市场营销/推广。这一新工具将提供更多独特的数据，使 研究人员最好地推进他们的计划，以获得最大的影响。

ABSTRACT Funds are requested for a NanoString GeoMxTM Digital Spatial Profiler (DSP). This instrument is a multiplexed imaging device that facilitates spatial gene expression analysis. This instrument will provide unique value added to investigators at the Roswell Park Comprehensive Cancer Center and the surrounding area in Western New York. The DSP instrument couples technologies that give spatial information in formalin fixed tissue with downstream molecular analysis. Traditional technologies such as immunohistochemistry (IHC) or immunofluorescence (IF) provide spatial information but are limited by fluorophores. In contrast, single cell sequencing provides deep information on single cells, but spatial information is lost. The DSP can accurately and sensitively detect up to 1800 RNA molecules and 96 proteins, with spatial resolution using a combination of RNA probes and antibodies. This form of data is particularly relevant in the context of cancer research (e.g. tumor heterogeneity, tumor microenvironment), as well as other biological disciplines (e.g. developmental biology, neuroscience). While there are tools for spatial analyses and genomics at Roswell Park, there is no comparable technology to the DSP in a 200-mile radius. The instrument will be placed in the Center for Personalized Medicine (CPM), which is focused on advanced tissue analysis and juxtaposed with the Genomics Shared Resource (GSR) of Roswell Park which is focused on molecular analyses. This structure provides the unique combination of pathological and genomic expertise to appropriately leverage the DSP instrument. There are 8 major users of the instrument that combined will use ~80% of the instrument capacity. Remaining instrument capacity will be made available to internal or external researchers through our on-line scheduling and booking system. The CPM/GSR directors will generate a business plan, with a multidisciplinary advisory board responsible for instrument oversight, while the institution will support the instrument through service contracts, personnel, and marketing/promoting. This new instrument will provide more unique data that will enable funded researchers to best advance their programs for maximum impact.