Interactions Discovery Core

交互发现核心摘要 互动发现核心(IDC)将继续支持以细胞蛋白质为重点的互动项目 介导HIV-1感染早期事件的复合体。国际数据中心开展的项目将涉及多个 该中心的组成部分以及外部调查人员。目标1建议识别结合的细胞蛋白 特别是阳性选择下的HIV-1衣壳部位。这些高度协作的实验将是 由蛋白质核心和项目3支持，该项目将提供重组CA和/或可溶低聚CA 组装，并与艾滋病毒病毒学核心合作进行，这将验证病毒学高 优先命中。病毒学上相关的靶标随后将进入PCHPI结构确定 输油管道。目标2建议确定CPSF6相互作用的蛋白质，这些蛋白质介导HIV-1整合到基因中； 候选的CPSF6功能性合作伙伴将在A.Engelman的实验室进行进一步调查。目标3建议 在DNA修复和复制途径中识别新的HIV VPR靶点，特别是那些负责 VPR诱导的DNA损伤检查点。后面的实验将在合作中进行 项目6，它将验证命中，从而将继续提供新VPR的身份 与细胞蛋白质的复合体，用于PCHPI的机制和结构研究。因此，总体来说，IDC是 在PCHPI正在进行的科学的许多方面是完全综合的，并且是核心的。

Interactions Discovery Core Abstract The Interactions Discovery Core (IDC) will continue to support interactive projects focusing on cellular protein complexes mediating early events in HIV-1 infection. The projects carried out by the IDC will involve multiple components of the Center as well as outside investigators. Aim 1 proposes to identify cellular proteins binding specifically to HIV-1 capsid sites under positive selection. These highly collaborative experiments will be supported by the Protein Core and Project 3, which will provide recombinant CA and/or soluble oligomeric CA assemblies, and performed in collaboration with the HIV Virology Core, which will validate virologically high priority hits. The virologically relevant targets will subsequently enter the PCHPI structure determination pipeline. Aim 2 proposes to identify CPSF6 interacting proteins that mediate HIV-1 integration into genes; the candidate functional CPSF6 partners will be investigated further in A. Engelman's lab. Aim 3 proposes to identify novel HIV Vpr targets in DNA repair and replication pathways, and in particular those responsible for the induction of DNA damage checkpoint by Vpr. The latter experiments will be performed in collaboration with Project 6, which will validate the hits, and thereby will continue to provide the identity of new Vpr complexes with cellular proteins for mechanistic and structural studies in the PCHPI. Thus, overall, the IDC is completely integrated and central to many aspect of science being pursued at PCHPI.