Research Supplement for Kennelia Mellanson

摘要 在这里，我们向NIA申请我们R01拨款的研究补充，提交它是为了回应 PA-21-071，旨在促进科学研究人员的多样性，并制定 合格调查员的职业生涯。我们提出了一个详细的计划，以推进职业生涯 实验室的研究生Kennelia Mellanson说。Kennelia目前的项目专注于小说 确定脑室和淋巴系统关键成分的定量方法 脑内的运输--纤毛跳动，脑脊液(CSF)的定向流动，以及 脑室管膜层的结构--对有效的脑室转运至关重要 以及水通道蛋白4(AQP4)通道的结构，这是 灵敏的运输系统。 我们的建议完全符合美国国家航空航天局“淋巴和脑脊液系统”的R01拨款 《在衰老和阿尔茨海默病中》，其重点是脑组织动态平衡的机制 阿尔茨海默氏症。我们现在建议使用我们在脑室的新技术和发现 和淋巴转运来确定伴随着衰老和进展的变化 阿尔茨海默病小鼠模型，并利用这个项目作为帮助和指导的平台 Kennelia Mellanson在发展她作为研究人员和学者的职业生涯中。我们为您呈现一份详细的 Kennelia的职业发展计划，包括详细的计划和指导时间表， 手稿和助学金的撰写，授课活动，提高演示技巧，参与 会议和课程，以及网络。此外，我们还提出了研究关键字的研究计划 运输系统的组成部分，可能会因衰老和阿尔茨海默病而受损。

ABSTRACT Here we apply for a research supplement to our R01 grant from the NIA, submitting it in response to PA-21-071 which is directed at fostering diversity in scientific research workforce and developing the careers of eligible investigators. We present a detailed plan to advance the professional career of Kennelia Mellanson, a graduate student in the laboratory. Kennelia’s current project focuses on novel quantitative approaches to determine the key components of the ventricular and glymphatic system of transport in the brain – the ciliary beat, the directional flow of the cerebrospinal fluid (CSF), and the structure of the brain ependymal layer – features that are critical for the efficient ventricular transport of the CSF, and the structure of the aquaporin 4 (AQP4) channels, which serve as the key components of the glymphatic system of transport. Our proposal is in full alignment with the original R01 grant from the NIA “Glymphatic and CSF systems in aging and Alzheimer’s disease”, which focuses on the mechanisms of the brain tissue homeostasis and clearance in Alzheimer's. We now propose using our new techniques and findings on the ventricular and glymphatic transport to determine the changes accompany aging and progression of the Alzheimer's disease in a mouse model and to use this project as a platform for helping and directing Kennelia Mellanson in developing her career as a researcher and a scholar. We present a detailed plan for Kennelia’s professional development, including a detailed plan and timeline for mentorship, manuscript and grant writing, didactic activities, improving the presentation skills, participation in meetings and courses, and networking. Furthermore, we present a research plan for studying the key components of the transport systems that may be compromised by aging and by Alzheimer's disease.