Analytical Cell Models Core

分析单元模型核心-抽象 DDRCC分析单元模型核心建议为DDRCC成员提供访问最先进技术的途径 消化系统和肝病的组织培养模型。我们将使用基因组工程技术，包括 CRIPSR-CAS9将基因改变引入已建立的细胞系和诱导的多能干细胞 (IPSCS)。IPSCs将被分化成胃肠道和肝脏的细胞，用于 由发改委成员进行研究。核心还将为成员提供主要的实质性和非实质性的 实质肝细胞来自人和啮齿动物肝脏的细胞，以及用于细胞分析的关键工具。在……里面 除了向DDRCC成员提供可用于直接分析的细胞系外，核心还将 为成员提供培训和关键试剂，使他们能够在以下方面建立许多程序 他们自己的实验室。核心将采用一项严格的评估计划，以确保其执行 以最佳方式，并继续向发改委成员提供在新技术开发过程中获得这些技术的机会。Dr。 将领导核心的邓肯是干细胞生物学和细胞分化方面的专家，他使用了基因组 广泛地进行工程，以产生细胞来模拟肝病。他以前有过经营类似的 科尔是威斯康星医学院的教授。他将得到唐·罗基博士的支持 在从人和啮齿动物肝脏中分离细胞方面有丰富的经验。

Analytical Cell Models Core - Abstract The DDRCC Analytical Cell Models Core proposes to provide DDRCC members with access to state-of-the-art tissue culture models of digestive and liver disease. We will use genome engineering technologies including CRIPSR-Cas9 to introduce genetic alterations into established cell lines and into induced pluripotent stem cells (iPSCs). The iPSCs will be differentiated to form cells of the gastrointestinal tract and liver that will be used for study by DDRCC members. The Core will also provide members with primary parenchymal and non- parenchymal livers cells from both human and rodent livers as well as critical tools for the analyses of cells. In addition to providing DDRCC members with cell lines that can be used for direct analyses, the Core will also provide members with training and critical reagents that will allow them to establish many of the procedures in their own laboratories. The Core will adopt a program of rigorous evaluation that will ensure that it performs optimally and continues to provide DDRCC members access to new technologies as they are developed. Dr. Duncan, who will lead the core, is an expert in stem cell biology and cell differentiation, and he has used genome engineering extensively to generate cells to model liver disease. He has previous experience in running a similar core while a Professor at the Medical College of Wisconsin. He will be supported by Dr. Don Rockey who has extensive experience in the isolation of cells from both human and rodent livers.