NCCAT: National Center for CryoEM Access and Training

项目总结/摘要 位于纽约结构生物学中心的国家冷冻EM获取和培训中心（NCCAT）， 提供最先进的设备，包括标本制备机器人、高端显微镜 直接探测器，筛选显微镜，以及所有其他辅助设备， 使用冷冻电子显微镜（cryoEM）方法获得最高分辨率。一个高素质的技术 团队提供直接的支持、指导和帮助，以确保最高质量的结构 在最短的时间内生产。交叉培训计划为各种用户提供培训 技能水平和职业目标。 这是对国家冷冻EM获取和培训中心（NCCAT）的补充资金申请， 购买计算基础设施，以支持中心的增长和扩展能力。我们 正在申请资金，以购买三台实时处理8 GPU服务器，每台服务器配备30 TB SSD存储， 实时传入显微照片，以便提供有关样品/网格质量、蛋白质 组成和重建质量。实时处理将提供信息，使NCCAT用户和 显微镜操作员，以最大限度地提高效率和利用显微镜的时间。

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. This is a request for supplemental funding for the National Center for CryoEM Access and Training (NCCAT) to purchase computational infrastructure that will support the growth and expand the capabilities of the center. We are requesting funds for three live processing 8-GPU servers each with 30 TB SSD storage that will process incoming micrographs on-the-fly, in order to provide real-time feedback on sample/grid quality, protein composition, and reconstruction quality. Live processing will provide information that enables NCCAT users and microscope operators to maximize the efficiency and use of microscope time.