Bloomington Drosophila Stock Center at Indiana University

项目摘要 布鲁明顿果蝇种群中心(BDSC)收集、管理、维护和分发果蝇品系 黑腹果蝇支持生物医学研究社区。它是世界上最大、最大的 世界上全面的果蝇种群收集，它是许多研究成功的核心 项目--包括820多项国家卫生研究院现有赠款。COVID大流行导致了BDSC的几个方面 行动将受到影响，这里要求的资金将用于修复损坏和恢复正常 在信息系统管理、质量控制和研究领域通过聘用 印第安纳大学内部现有专业人员的临时服务。第一，签约电脑 规划服务将恢复正在进行的BDSC信息过渡项目的适当进展 将管理扩展到基于Web的平台。不幸的是，这个项目因转移了 BDSC的科学家致力于灾害管理。这些服务将有助于推动该项目在 对所有BDSC功能至关重要的遗留软件不再可用。其次，短期员工将允许 BDSC将重新调配经验丰富的库存管理人员的工作，以赶上对库存的监控 交叉污染或基因崩溃在COVID限制仅强制执行时被暂停 校园里的基本工作。确定有问题的库存对于确保样本调查人员 接受是值得信赖的。最后，一位经验丰富的技术人员将致力于资源开发项目 产生研究肠道上皮细胞功能所需的菌株。父级赠款支持 可靶向表达UA的GAL4和Split-GAL4驱动因子的鉴定与鉴定 转基因存在于特定的中肠细胞中，但大流行阻碍了预期的进展速度。具体地说，它 推迟向研究发展的科学家引入新的、更有效的驱动因素 肠内分泌细胞的功能，这是一种与人类同源的模拟多肽激素分泌细胞类型。 总之，这些干预措施将修复COVID造成的操作缺陷，并使BDSC能够 以大流行前存在的高质量为生物医学研究人员提供服务。

Project Summary The Bloomington Drosophila Stock Center (BDSC) collects, curates, maintains and distributes strains of the fly Drosophila melanogaster to support the biomedical research community. It is the largest and most comprehensive Drosophila stock collection in the world, and it is central to the success of many research projects—including over 820 active NIH grants. The COVID pandemic has caused several aspects of BDSC operations to suffer, and the funding requested here will serve to repair damage and restore normal productivity in the areas of information system management, quality control and research by engaging the temporary services of available professionals within Indiana University. First, contracted computer programming services will restore appropriate progress to an ongoing project transitioning BDSC information management to a web-based platform. Unfortunately, this project was slowed irreparably by diverting effort of BDSC scientists to disaster management. These services will help push the project to completion before legacy software critical to all BDSC functions is no longer usable. Second, short-term employees will allow the BDSC to redeploy the effort of experienced stockkeeping staff to catch up on the surveillance of stocks for cross-contamination or genetic breakdown that was suspended when COVID restrictions mandated only essential work on campus. Identifying problematic stocks is critical to assuring that the samples investigators receive are trustworthy. Finally, an experienced technician will devote effort to a resource-development project generating strains needed for investigating intestinal epithelial cell functions. The parent grant supports the identification and characterization of GAL4 and split-GAL4 drivers that allow targeted expression of UAS transgenes in specific midgut cells, but the pandemic prevented the expected rate of progress. Specifically, it delayed the introduction of new, more-effective drivers to scientists investigating the development and functions of enteroendocrine cells, a model peptide hormone-secreting cell type with human cognates. Altogether, these interventions will repair COVID-inflicted operational deficiencies and allow the BDSC to provide services to biomedical researchers at the high level of quality that existed before the pandemic.