Selective Control of Synaptically-Connected Circuit Elements by Interluminescence - Diversity Supplement: E. CRESPO

项目摘要/摘要 多样性补充剂候选人将接受在体内验证内发光的所有方面的培训 急性细胞外记录和计算复杂的电生理分析。实验性的 方法将允许候选人使用定义明确的实验模型来评估相互发光 大脑皮层动力学。 应聘者将获得的培训经验对于他理解、设计和解释 最先进的神经数据。培训计划将补充他之前在分子和蛋白质方面的广泛培训 细胞神经科学。根据在体内应用化学遗传和光遗传工具的经验， 候选人将有一个非常强大的背景，以实现他的职业目标。他的训练范围很广 从在工程蛋白质水平上开发新探针的实践经验到它们的预期用途 在控制神经活动和行为方面。因此，这种体内电生理学的训练不仅会 对父母的资助有很大贡献，但会特别为他提供解剖所需的经验 新大脑皮层动力学，并在工具开发流水线上“循环”。 考虑到应聘者卓有成效的记录，申请表中列出的拟议培训是理想的。一个 两位导师之间建立的团队培训策略将培养候选人独立工作的能力 研究突触连接电路的作用。 拟议的补充活动将在原范围内增加父奖项的整体影响 奖励，同时推进候选人的培训和职业发展。

PROJECT SUMMARY/ABSTRACT The Diversity Supplement candidate will be trained in all aspects of validating Interluminescence in vivo with acute extracellular recordings and computational sophisticated electrophysiological analysis. The experimental approach will allow the candidate to assess Interluminescence with a well-defined experimental model to dissect cortical dynamics. The training experience the candidate will gain are critical for his understanding, designing, and interpretation of state-of-the-art neural data. The training plan will complement his prior extensive training in molecular and cellular neuroscience. Following this experience in applying chemogenetic and optogenetic tools in vivo, the candidate will have an exceedingly strong background for meeting his career goals. His training will have ranged from hands-on experience developing novel probes at the level of the engineered protein, to their intended use in controlling neural activity and behavior. Therefore, this training in in vivo electrophysiology will not only significantly contribute to the parent grant but will specifically provide him the experience required to dissect neocortical dynamics and come “full circle” in the tool development pipeline. The proposed training outlined in the application is ideal given the candidate’s productive track record. An established team training strategy between the two mentors will foster the candidate’s ability to independently investigate the role of synaptically-connected circuits. The proposed supplement activities will increase the parent award’s overall impact within the original scope of award while at the same time advancing the training and career development of the candidate.