Zebrafish International Resource Center

项目摘要 斑马鱼是研究脊椎动物发育、生理、行为、遗传和 疾病强大的技术可以有效地产生和恢复影响基因的斑马鱼突变 调节发育模式、器官形成、生理和行为。最新进展使其 易于在转基因斑马鱼和反义寡核苷酸中研究基因功能。的功能 这些基因中的许多（如果不是大多数的话）在脊椎动物群体中是保守的。因此，分析斑马鱼 突变提供了对包括人类在内的其他脊椎动物的基因功能的见解。 斑马鱼国际资源中心（ZIRC）是一个储存库， 转基因动物，材料和服务的研究界。父母补助金提供 资助1）作为斑马鱼遗传资源和研究材料的中央储存库，2）提供 病理学服务和咨询，以及3）开发一个平台，基于一组PCR检测， 筛选实验室斑马鱼最常见的病原体。这些材料、服务和信息将 向研究界广泛提供。 自成立以来，ZIRC在进口时花费了大量时间和资源用于设施的生物安全 鱼.我们还维持一个广泛的系统，以监测我们的股票的健康，以限制两个蔓延， 最常见的病原体，嗜神经假瘤和龟分枝杆菌。请求的补充 资金将用于改造和翻新设施，使我们能够消除P. neurophilia感染， 野生型股票。我们将改变食物准备室和主要鱼类设施的一部分，我们将 购买水过滤、鱼舍、食品房和PCR诊断设备。设备和空间 这些改变将支持筛选、饲养并最终维持没有嗜神经性丙酸杆菌的种群 并且M的患病率显著较低。龟类。

PROJECT SUMMARY Zebrafish is a premiere organism to study vertebrate development, physiology, behavior, genetics, and disease. Powerful techniques allow efficient generation and recovery of zebrafish mutations affecting genes that regulate developmental patterning, organogenesis, physiology and behavior. Recent advances make it easy to study gene function in transgenic zebrafish and with antisense oligonucleotides. The functions of many, if not most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene function in other vertebrates, including humans. The Zebrafish International Resource Center (ZIRC) has been established as a repository that provides genetically modified animals, materials, and services to the research community. The parent grant provides funding 1) to serve as a central repository for zebrafish genetic stocks and research materials, 2) to provide pathology services and consultations, and 3) to develop a platform, based on a panel of PCR assays, to screen for the most prevalent pathogens of laboratory zebrafish. These materials, services, and information will be made widely available to the research community. Since its inception, ZIRC expends considerable time and resources on the facility's biosecurity when importing fish. We also maintain an extensive system to monitor the health of our stocks to limit the spread of two of the most common pathogens, Pseudoloma neurophilia and Mycobacterium chelonae. The requested supplemental funds will be used to alter and renovate the facility so that we can eliminate P. neurophilia infections in our wild-type stocks. We will alter the food preparation room and parts of the main fish facility, and we will purchase water filtration, fish housing, food room, and PCR diagnostic equipment. The equipment and space alterations will support screening for, rearing, and ultimately maintaining stocks that are free of P. neurophilia and have significantly lower prevalence of M. chelonae.