Stanford Islet Research Core

斯坦福大学小岛研究核心(SIRC)：项目摘要/摘要 国家发改委小岛研究核心(SIRC)的专业知识为国家发改委调查人员提供了 对高质量的胰岛和胰腺进行现代分子、细胞和功能研究的能力 啮齿动物和人类。国家发改委的一大批合作调查人员研究了广泛的岛屿 生理或病理环境中的生物学，包括胰岛发育、功能成熟、 维持成熟细胞的命运、增殖、遗传学、表观遗传学、基因调控、器官间信号传递、 胰岛细胞内信号转导、胰岛免疫学和衰老。SIRC的一个中心方面是我们直接关注的 在啮齿动物和人类组织中的平行研究。支持大多数研究的重要组成部分 属于这一范例的是我们分离高质量、特征良好的啮齿动物或人类原生动物的能力 进行胰岛细胞表型、分子表型和生理表型分析。这是 再加上一个可靠、强有力的计划，以获得高质量的人类胰岛，从而增加了 国家发改委研究人类胰岛生物学的调查人员。基于移植的人胰岛研究的支持 SIRC的细胞功能、生长和命运也加强了对人类胰岛的潜在翻译研究 糖尿病。除了这些服务外，SIRC还致力于对调查人员进行专门方法培训 胰岛生物学，如胰岛分离和移植，胰岛培养和包括胰岛素或 胰高血糖素酶联免疫吸附试验、胰岛灌注法和静态批量胰岛素分泌试验、免疫组织学检测。SIRC 工作人员与国家发改委调查人员密切合作，根据他们的目标制定有效的实验策略。 如果没有P30对这项独特的斯坦福研究的支持，这些以小岛为重点的服务或活动将会丢失 核心。SIRC还通过不断开发新的 实验能力，以满足现代岛屿调查的动态需求。这包括化验 胰岛功能，研究人类胰岛细胞遗传学的新方法，胰岛移植的新模型，以及 帮助斯坦福大学医疗保健中心开发新的临床胰岛项目，如斯坦福大学胰岛 替代和免疫耐受(SPIRIT)临床胰岛移植计划。SIRC还增强了 通过将活动与其他发改委研究核心结合起来，使用这些核心。与糖尿病并存 免疫监测核心(DIMC)和糖尿病基因组与分析核心(DGAC)，SIRC合作 关于允许对人类胰腺进行细胞、遗传、分子、生理和基因组研究的努力-以及 免疫细胞或血细胞，包括免疫细胞、基质细胞和血管细胞。SIRC还将为国家发改委提供服务 加州大学伯克利分校或加州大学戴维斯分校的成员，包括通过 建议扩大区域试点和可行性奖。基于发改委成员的增长，演变 令人兴奋的新服务，不断增加的人类小岛会员需求，以及更多的研究 专注于发改委的小岛生物，我们预计未来几年对SIRC服务的需求将会增长。

STANFORD ISLET RESEARCH CORE (SIRC): PROJECT SUMMARY/ABSTRACT The specialized expertise of the SDRC Islet Research Core (SIRC) provides SDRC investigators with the capacity to perform modern molecular, cellular and functional studies of high-quality islets and pancreata from rodents and humans. The SDRC’s large group of collaborative investigators study a broad spectrum of islet biology in physiological or pathological settings, including islet development, functional maturation, maintenance of mature cell fate, proliferation, genetics, epigenetics, gene regulation, inter-organ signaling, intra-islet cell signal transduction, islet immunology, and aging. A central aspect of the SIRC is our direct focus on parallel studies in rodent and human tissues. An essential component supporting the majority of studies falling under this paradigm is our ability to isolate high-quality, well characterized rodent or human primary pancreatic islets, and then to perform assays of islet cellular, molecular and physiological phenotypes. This is coupled with a reliable, robust program to procure high-quality human islets that have increased the number of SDRC investigators studying human islet biology. The support of transplantation-based studies of human islet cell function, growth and fate by the SIRC also enhances potential translational studies of human islets for diabetes. In addition to these services, the SIRC is committed to training investigators in specialized methods of islet biology, like islet isolation and transplantation, islet culture and specialized assays including insulin or glucagon ELISA, islet perifusion and static batch insulin secretion assays, and immunohistology. SIRC personnel work closely with SDRC investigators to build efficient experimental strategies tailored to their aims. These islet-focused services or activities would be lost without P30 support of this unique Stanford research core. The SIRC also serves the Stanford community and the SDRC by continuously developing new experimental capacity to match the dynamic demands of modern islet investigations. This includes assays of islet function, new approaches to investigate human islet cell genetics, new models of islet transplantation, and assistance in developing new clinical islet programs at Stanford Health Care, like the Stanford Pancreatic Islet Replacement and Immune Tolerance (SPIRIT) clinical islet transplantation programs. The SIRC also enhances the use of other SDRC Research Cores by integrating activities with those cores. Together with the Diabetes Immune Monitoring Core (DIMC) and the Diabetes Genomics & Analysis Core (DGAC), the SIRC collaborates on efforts permitting cellular, genetic, molecular, physiological and genome studies of human pancreas- and immune or blood cells, including immune, stromal and vascular cells. The SIRC will also serve SDRC members at University of California (UC) Berkeley or at UC Davis, including those supported through the proposed Regional Pilot & Feasibility Award expansion. Based on growth of the SDRC membership, evolution of exciting new services, increasing membership demand for human islets, and the increased research focused on islet biology in the SDRC, we anticipate growth of demand for SIRC services in the coming years.