Islet Procurement and Research Core

项目摘要/摘要(小岛核心) 小岛采购和研究中心(IPRC)的专门小岛生物学专业知识将为SDRC提供 有能力进行高质量的现代分子、细胞和功能研究的研究人员 啮齿动物和人类的胰岛和胰腺。发改委的一大批合作调查人员研究了一项 生理或病理环境中广泛的胰岛生物学，包括胰岛发育、功能 成熟、维持成熟细胞命运、增殖、遗传学、表观遗传学、基因调控、器官间 信号、胰岛细胞内信号转导、胰岛免疫学和衰老，仅举几例。的一个中心方面 IPRC是我们在啮齿动物和人类组织中进行平行研究的直接重点。一个必不可少的组成部分 支持这一范式下的大多数研究的是我们分离出高质量、良好的 鉴定啮齿动物胰岛，并进行胰岛细胞、分子和生理分析 表型。这与IPRC中可靠的、强有力的方案相结合，以获得高质量的人类小岛 这增加了国家发改委研究人类胰岛生物学的调查人员的数量。的支持 IPRC对人类胰岛细胞功能、生长和命运的基于移植的研究也将得到加强 人类胰岛治疗糖尿病的潜在翻译研究。除了这些服务外，IPRC还致力于 对研究人员进行胰岛生物学专门方法的培训，如胰岛分离和移植 培养和专门化检测，包括胰岛素酶联免疫吸附试验、胰升糖素酶联免疫吸附试验、灌注法和静态间歇胰岛素法 分泌物分析和免疫组织学检查。IPRC人员将与国家发改委调查人员密切合作，建立 根据他们的目标量身定做的有效的实验策略。IPRC还为斯坦福社区提供服务 科学与发改委不断发展新的实验能力以适应动态需求 现代岛屿调查的最新进展。这包括胰岛功能的分析，研究人类胰岛的新方法 细胞遗传学，胰岛移植的新模式，以及在开发新的临床胰岛计划方面的帮助 斯坦福医疗保健公司的医院和诊所。创新研究中心亦加强其他研究核心的使用。 斯坦福大学民主联盟。与糖尿病免疫监测核心(DIMC)和糖尿病基因组学一起& 分析核心(DGAC)，IPRC将整合允许细胞、遗传、分子、生理和 与糖尿病相关的人胰腺和胰岛相关细胞的基因组研究，包括免疫、间质 和血管细胞。总而言之，小岛采购和研究核心将作为一种无价的、新颖的 为国家发改委提供资源，为调查人员提供针对胰腺和胰岛的专门知识和培训，以 支持和扩大他们与糖尿病相关的研究。

Project Summary / Abstract (Islet Core) The specialized islet biology expertise of the Islet Procurement and Research Core (IPRC) will provide SDRC investigators with the capacity to perform modern molecular, cellular and functional studies of high-quality islets and pancreata from rodents and humans. The SDRC's large group of collaborative investigators study a broad spectrum of islet biology in physiological or pathological settings, including islet development, functional maturation, maintenance of mature cell fate, proliferation, genetics, epigenetics, gene regulation, inter-organ signaling, intra-islet cell signal transduction, islet immunology, and aging, to name a few. A central aspect of the IPRC is our direct focus on parallel studies in rodent and human tissues. An essential component supporting the majority of studies falling under this paradigm is our ability to isolate high-quality, well- characterized rodent pancreatic islets, and then to perform assays of islet cellular, molecular and physiological phenotypes. This is coupled with a reliable, robust programs in the IPRC to procure high-quality human islets that have increased the number of SDRC investigators studying human islet biology. The support of transplantation-based studies of human islet cell function, growth and fate by the IPRC will also enhance potential translational studies of human islets for diabetes. In addition to these services, the IPRC is committed to training investigators in specialized methods of islet biology, like islet isolation and transplantation, islet culture and specialized assays including insulin ELISA, glucagon ELISA, perfusion and static batch insulin secretion assays, and immunohistology. IPRC personnel will work closely with SDRC investigators to build efficient experimental strategies tailored to their aims. The IPRC also serves the Stanford community of science and the SDRC by continuously developing new experimental capacity to match the dynamic demands of modern islet investigations. This includes assays of islet function, new approaches to investigate human islet cell genetics, new models of islet transplantation, and assistance in developing new clinical islet programs at the hospitals and clinics of Stanford Health Care. The IPRC also enhances the use of other Research Cores in the Stanford DRC. Together with the Diabetes Immune Monitoring Core (DIMC) and the Diabetes Genomics & Analysis Core (DGAC), the IPRC will integrate efforts permitting cellular, genetic, molecular, physiological and genome studies of human pancreas- and islet-associated cells relevant to diabetes, including immune, stromal and vascular cells. In summary, the Islet Procurement and Research Core will serve as an invaluable, novel resource for the SDRC, providing investigators with the pancreas- and islet-specific expertise and training to enable and expand their diabetes-related research.