Islet Procurement and Research Core

项目概要/摘要（胰岛核心） 胰岛采购和研究核心（IPRC）的专业胰岛生物学知识将为SDRC提供 具有进行高质量的现代分子，细胞和功能研究的能力的研究人员 从啮齿动物和人类的胰岛和胰腺。SDRC的大型合作调查小组研究了一个 生理或病理环境中的广谱胰岛生物学，包括胰岛发育、功能 成熟，成熟细胞命运的维持，增殖，遗传学，表观遗传学，基因调控，器官间 信号传导、胰岛细胞内信号转导、胰岛免疫学和衰老，仅举几例。的一个核心方面 IPRC是我们直接关注啮齿动物和人体组织平行研究。一个重要组成部分 支持大多数属于这种范式的研究是我们能够分离出高质量的，良好的， 表征啮齿类动物胰岛，然后进行胰岛细胞、分子和生理的测定， 表型这与IPRC中可靠，强大的程序相结合，以获得高质量的人类胰岛 这增加了SDRC研究人类胰岛生物学的研究人员的数量。的支持 IPRC对人类胰岛细胞功能、生长和命运的基于移植的研究也将提高 人类胰岛对糖尿病的潜在转化研究。除了这些服务外，IPRC还致力于 培养胰岛生物学专门方法的研究人员，如胰岛分离和移植，胰岛 培养和专门的测定，包括胰岛素ELISA、胰高血糖素ELISA、灌注和静态批次胰岛素 分泌测定和免疫组织学。IPRC人员将与SDRC调查人员密切合作， 为他们的目标量身定制的有效实验策略。IPRC还为斯坦福大学社区提供服务， 通过不断开发新的实验能力来满足动态需求， 现代小岛研究的一部分这包括胰岛功能的测定，研究人类胰岛功能的新方法， 细胞遗传学，胰岛移植的新模型，并协助开发新的临床胰岛计划， 斯坦福大学医疗保健的医院和诊所。IPRC还加强了其他研究核心的使用， 斯坦福大学DRC。与糖尿病免疫监测核心（DIMC）和糖尿病基因组学和 分析核心（DGAC），IPRC将整合努力，允许细胞，遗传，分子，生理和 与糖尿病相关的人类胰腺和胰岛相关细胞的基因组研究，包括免疫、间质 和血管细胞。总之，胰岛采购和研究核心将作为一个宝贵的，新颖的， SDRC的资源，为研究者提供胰腺和胰岛特异性专业知识和培训， 促进并扩大他们的糖尿病相关研究。

Project Summary / Abstract (Islet Core) The specialized islet biology expertise of the Islet Procurement and Research Core (IPRC) will provide SDRC investigators with the capacity to perform modern molecular, cellular and functional studies of high-quality islets and pancreata from rodents and humans. The SDRC's large group of collaborative investigators study a broad spectrum of islet biology in physiological or pathological settings, including islet development, functional maturation, maintenance of mature cell fate, proliferation, genetics, epigenetics, gene regulation, inter-organ signaling, intra-islet cell signal transduction, islet immunology, and aging, to name a few. A central aspect of the IPRC is our direct focus on parallel studies in rodent and human tissues. An essential component supporting the majority of studies falling under this paradigm is our ability to isolate high-quality, well- characterized rodent pancreatic islets, and then to perform assays of islet cellular, molecular and physiological phenotypes. This is coupled with a reliable, robust programs in the IPRC to procure high-quality human islets that have increased the number of SDRC investigators studying human islet biology. The support of transplantation-based studies of human islet cell function, growth and fate by the IPRC will also enhance potential translational studies of human islets for diabetes. In addition to these services, the IPRC is committed to training investigators in specialized methods of islet biology, like islet isolation and transplantation, islet culture and specialized assays including insulin ELISA, glucagon ELISA, perfusion and static batch insulin secretion assays, and immunohistology. IPRC personnel will work closely with SDRC investigators to build efficient experimental strategies tailored to their aims. The IPRC also serves the Stanford community of science and the SDRC by continuously developing new experimental capacity to match the dynamic demands of modern islet investigations. This includes assays of islet function, new approaches to investigate human islet cell genetics, new models of islet transplantation, and assistance in developing new clinical islet programs at the hospitals and clinics of Stanford Health Care. The IPRC also enhances the use of other Research Cores in the Stanford DRC. Together with the Diabetes Immune Monitoring Core (DIMC) and the Diabetes Genomics & Analysis Core (DGAC), the IPRC will integrate efforts permitting cellular, genetic, molecular, physiological and genome studies of human pancreas- and islet-associated cells relevant to diabetes, including immune, stromal and vascular cells. In summary, the Islet Procurement and Research Core will serve as an invaluable, novel resource for the SDRC, providing investigators with the pancreas- and islet-specific expertise and training to enable and expand their diabetes-related research.