Genomics

项目总结 基因组学共享资源(G-SR)是一个最先进的基因组学设施，致力于提供最新的 低成本的基因组研究工具。G-SR的主要重点是促进高影响力的癌症研究 由SCI成员通过提供最先进的基于遗传和基因组的工具和标准化分析 使用这些工具。这一目标是通过为SCI成员提供不受阻碍的、全面的、 在快速周转的时间内以高接触、专家的方式获得这些基因组技术 咨询服务，涵盖基因组学实验的方方面面，从样品制备到计算 数据分析。主要的G-SR技术和服务包括高通量测序、单细胞基因组 分析，空间基因组分析，基于微阵列的分析，数字和实时定量聚合酶链式反应，多重 单分子分析，以及这些技术所需的所有样品制备服务。专家指导 随着每一项技术的使用，还提供了贯穿整个实验过程的指导 具有实验设计、数据分析、数据归档和发表等功能。斯坦福大学以前的资源 功能基因组学设施(SFGF)、遗传学测序服务中心(GSSC)、蛋白质和核 ACID设施(PAN)和遗传学生物信息学服务中心(GBSC)现已合并到 在John Coller博士的领导下，作为G-SR总监，提供全方位的服务。迈克尔·斯奈德 博士(PS)，是教员顾问。所有计划的成员都经常使用G-SR，其中使用最频繁的是 来自癌症生物学、癌症干细胞和癌症免疫治疗计划。G-SR做出了贡献 至46份以癌症为重点的出版物(30份高影响力出版物)。G-SR的年度预算为3,652,748美元，而CCSG 要价是183,830美元。因此，G-SR利用广泛的机构支持，只寻求5%的 CCSG基金。G-SR专注于以下主要科学目标：(1)提供最先进的基因组学 能力，(2)促进开发新的实验方法和技术，(3)评估 实施用于癌症研究的尖端基因组学技术，以及(4)提供基因组学培训 技术、实验分析和分析方法。G-SR的未来计划包括扩大癌症- 专门的生物信息学支持，增加了一名全职生物信息学家，扩大了高通量 测序和单细胞基因组能力。

PROJECT SUMMARY The Genomics Shared Resource (G-SR) is a state-of-the-art genomics facility dedicated to providing the latest genomic research tools at a low cost. The primary focus of the G-SR is to facilitate high-impact cancer research by SCI members by providing the most advanced genetic and genomic-based tools and standardized assays using these tools. This goal is accomplished by providing SCI members with unencumbered, comprehensive, and cost-effective access to these genomics technologies at rapid turn-around times with high-touch, expert consultation services that cover all aspects of genomics experiments from sample preparation to computational analysis of data. Major G-SR technologies and services include high-throughput sequencing, single-cell genomic analysis, spatial genomic analysis, microarray-based analysis, digital and real-time quantitative PCR, multiplex single-molecule analysis, and all sample preparation services required for these technologies. Expert guidance with the use of each technology is also provided throughout the entire experimental process, including guidance with experimental design, data analysis, data archiving and publication. The previous resources of the Stanford Functional Genomics Facility (SFGF), the Genetics Sequencing Service Center (GSSC), Protein and Nucleic Acid Facility (PAN), and Genetics Bioinformatics Service Center (GBSC) have been now combined under the leadership of John Coller, PhD, as the G-SR Director, to provide the full spectrum of services. Michael Snyder, PhD (PS), is the faculty advisor. Members of all programs regularly use the G-SR, with the heaviest use coming from the Cancer Biology and Cancer Stem Cells and Cancer Immunotherapy programs. The G-SR contributed to 46 cancer-focused publications (30 high-impact). The annual budget of the G-SR is $3,652,748, yet the CCSG request is $183,830. Accordingly, the G-SR leverages extensive institutional support and seeks only 5% from CCSG funds. The G-SR is focused on the following major scientific aims: (1) to provide state-of-the-art genomics capabilities, (2) to facilitate the development of novel experimental methods and technologies, (3) to evaluate and implement cutting-edge genomics technologies for cancer research, and (4) to provide training on genomics technologies, experimental assays, and analysis methods. Future plans of the G-SR include expanding cancer- specific bioinformatics support with the addition of one full-time bioinformatician, expanding high-throughput sequencing and single-cell genomic capabilities.