600 MHz NMR Spectrometer and CPMAS CryoProbe

项目总结： 我们建议购买第一个用于固态核磁共振的CPMAS低温探头，安装在 美国，以及一个专门的600 MHz核磁共振光谱仪，以支持生物分子研究 在加州大学河滨分校和周围的南加州地区。这件事的敏感性增加了 该工具将支持由美国国立卫生研究院资助的变革性研究项目，其中包括： 神经退行性变的异染色质形成与基因沉默、结构转化 疾病相关蛋白(淀粉样蛋白)，病原体的膜蛋白效应器与 依赖吡哆醛-5‘-磷酸的寄主、机械酶学和化学结构/动力学 酶，β-内酰胺酶的抑制，以及生物聚合物的机理。这些问题都有一个共同点 通常需要在实验灵敏度的极限下进行分子表征。尽管很好- 配备固态核磁共振，支持常规和动态核极化(DNP) 检测，目前在南加州或北美没有设施与 必须保持在房间内或房间附近的样品所需仪器的灵敏度 温度。因此，这种设备将填补核磁共振表征类型中的一个明显空白 这既可以在地区上进行，也可以在全国范围内进行。拟议的低温探测器和光谱仪将 成为加州大学河滨分校分析化学仪器的一部分 为校园、当地社区和来自全国各地的外部用户提供服务的设施。

Project Summary: We propose the acquisition of the first CPMAS cryoprobe for solid-state NMR to be housed in the United States, along with a dedicated 600 MHz NMR spectrometer to support biomolecular research at UC Riverside and the surrounding Southern California region. The increased sensitivity of this instrument will enable transformative research projects supported by NIH funding that include: heterochromatin formation and gene silencing, structural transformations of neurodegenerative disease related proteins (amyloids), membrane protein effectors of pathogen interactions with the host, mechanistic enzymology and chemical structure/dynamics of pyridoxal-5'-phosphate-dependent enzymes, inhibition of β-lactamases, and the mechanics of biopolymers. These problems all share a common need for molecular characterization at the limits of experimental sensitivity. Although well- equipped for solid-state NMR with conventional and dynamic nuclear polarization (DNP) enabled detection, there are currently no facilities within Southern California or North America with the sensitivity of the requested instrumentation for samples that must be maintained at or near room temperature. As such, this equipment will fill a noticeable void in the types of NMR characterization that can be performed both regionally and nationally. The proposed cryoprobe and spectrometer will become part of the University of California at Riverside’s Analytical Chemistry Instrumentation Facility, which serves the campus, local community, and external users from across the country.