Translational Pathology and Data Analysis Core (TPDAC)

摘要 实现每个翻译和基础科学的翻译和基础科学目标 腺瘤中心(TBAC)项目将依赖于可靠地获得生物标本、分析和数据服务 这将由翻译病理学和数据分析核心(TPDAC)提供。TPDAC促进了 威斯康星大学病理学系现有资源/设施的利用 病理学(TRIP)实验室和弗雷德·哈钦森的实验室医学转化研究倡议 癌症研究中心(FHCRC)以及UWI病理和医学系的教师专业知识 实验室医学、放射学、肿瘤学和生物统计学。种群之间的互补性在 两家公共研究机构将允许TBAC内部和内部的调查人员访问不会 不仅提高了我们对早期结肠病变的了解，而且还确保了患者多样性的充分代表 在我们的研究中。此外，TBAC U54项目研究需要使用创新技术的分析资源， 例如数字空间剖面图、单细胞序列和多光谱成像。技术的可获得性和 威斯康星大学TRIP实验室的专业技术可以在单细胞分辨率下对多个分子靶标进行定量分析 在一个单独的组织切片中。最后，TPDAC的承诺确保了大数据的一致实施 分析和统计，以加强TBAC的翻译目标，其中包括提供高维 用于跨标签协作研究的数据集。在U54的支持下，TPDAC将包含所需的专业知识 帮助识别、优化和实施早期结肠病变研究的最新技术。 TPDAC借鉴了生物显微镜科学、研究病理学、成像和数据分析的元素 华盛顿大学、UWCCC、FHCRC和华盛顿大学的服务单位。附加性和互补性专业知识 这两个研究机构的资源将能够为早期结肠损伤提供研究人员 在所有TBAC项目中，提供广泛的病理学和分析服务，以支持翻译和基础 研究。具体目标是： 目标1)收集、组织和分发来自计算机断层扫描的带注释的息肉和成像数据 结肠造影术(CTC)有助于早期结肠病变的研究。 目的2)支持非进展期结肠腺瘤、进展期结肠腺瘤和配对正常结肠腺瘤的多组学分析 来自ColoCare和GiCaRes队列的结肠样本。 目标3)支持空间和分子图谱项目，提供全面的病理学服务和 分析工具。 目的4)支持从多组学研究中产生的高维数据的分析和集成。

SUMMARY Accomplishment of the translational and basic science aims of each Translational and Basic Science of Adenomas Center (TBAC) project will depend on reliable access to the biospecimens, analytic and data services that will be provided by the Translational Pathology and Data Analysis Core (TPDAC). The TPDAC facilitates the utilization of existing resources/facilities in the University of Wisconsin (U WI) Department of Pathology & Laboratory Medicine Translational Research Initiatives in Pathology (TRIP) Laboratory and Fred Hutchinson Cancer Research Center (FHCRC) along with faculty expertise in the U WI Departments of Pathology & Laboratory Medicine, Radiology, Oncology and Biostatistics. The complementarity between the populations at two public research institutions will allow intra- and inter-TBAC investigators to access biospecimens that will not only advance our knowledge of early colonic lesions, but also ensure adequate representation of patient diversity in our studies. In addition, TBAC U54 Project studies require analytic resources that use innovative technologies, such as Digital Spatial Profiling, Single cell Seq, and multispectral imaging. The availability of technology and expertise in the UW TRIP Lab permits quantitative analysis of multiple molecular targets at single-cell resolution in a single tissue section. Finally, the commitment of the TPDAC ensures consistent implementation of big data analysis and statistics to enhance the TBAC’s translational goals, which include providing high-dimensional datasets for cross-TBEL collaborative studies. Under U54 support, the TPDAC will contain the expertise needed to help identify, optimize and implement the newest technology for early colon lesion research. The TPDAC draws upon elements from biospecimen science, research pathology, imaging and data analysis services units at U WI, UWCCC, FHCRC, and Univ of Washington. The additive and complementary expertise and resources at the two research institutions will be capable of providing investigators of early colonic lesions in all of the TBAC Projects with a wide array of pathology and analytic services to support translational and basic research. The Specific Aims are: Aim 1) To collect, organize, and distribute annotated polyps and imaging data from computed tomography colonography (CTC) to facilitate research in early colonic lesions. Aim 2) To support multi-omics analysis of nonadvanced and advanced colonic adenomas and matched normal colon samples from the ColoCare and GiCaRes cohorts. Aim 3) To support spatial and molecular profiling projects with a comprehensive array of pathology services and analytic tools. Aim 4) To support analysis and integration of high dimensional data generated from multi-omics studies.