FIBEROPTIC REFLECTANCE/PHOSPHORESCENCE SPECTROMETER

本研究的长期目标是开发一种基于光纤的 反射式血氧饱和度无创测定仪 细胞内的组织隔间。第一阶段成功演示了 利用曲线拟合法进行频谱分析的可行性 同时测定心外膜溶解氧和心外膜氧分压 心外膜组织中肌红蛋白和血红蛋白的脱氧形式。 这一方法将在第二阶段应用于制定 一种灵敏度更高的仪器来分解血红蛋白，肌红蛋白 和线粒体细胞色素。该设备的商业用途将 通过将其应用于工作哺乳动物的研究而得到证明 心脏和骨骼肌缺血的研究。要完成的任务 在第二阶段进行的工作将包括： 。确定曲线拟合反射率的最佳反射率标准 心外膜和骨骼肌的波谱 。构建光纤耦合成像光谱仪进行监测 同时在两个不同组织区域的组织氧 。同步评价组织氧监测仪的潜力 工作灌流时心外膜和心内膜氧合的监测 哺乳动物心脏 。通过使用该仪器进一步说明该仪器的实用性 骨骼肌缺血的磁共振研究

The long term goal of this research is to develop a fiberoptic-based reflectance instrument to noninvasively measure oxygen in circulatory and intracellular tissue compartments. Phase I successfully demonstrated the feasibility of employing curve fitting spectral analysis to simultaneously measure epicardial dissolved oxygen and the oxygenated and deoxygenated forms of myoglobin and hemoglobin in epicardial tissue. This methodology will be applied during Phase II to the development of an instrument with increased sensitivity to resolve hemoglobin, myoglobin and mitochondrial cytochromes. The commercial utility of the device will be demonstrated through its application to studies of working mammalian hearts and studies of ischemia in skeletal muscle. The tasks to be undertaken during Phase II will be to: . determine optimal reflectance standards for curve fitting reflectance spectra from epicardium and skeletal muscle . construct an optical fiber-coupled imaging spectrograph to monitor tissue oxygen at two different tissue regions simultaneously . evaluate the Tissue Oxygen Monitor's potential by simultaneously monitoring oxygenation of epicardium and endocardium in working perfused mammalian heart . further demonstrate the instrument's utility through its employment in a magnetic resonance study of skeletal muscle ischemia