UTERINE RECEPTIVITY TO TROPHOBLAST ADHESION

研究的目的是确定和表征 粘附所需的兔子宫表面特性 并与植入胚胎的滋养层表面融合。 的 要检验的假设是上皮表面是主要的 调节激素调节子宫容受性的部位， 相反的滋养层细胞和子宫细胞的顶膜 通过受体-配体相互作用粘附。 生物化学和结构 方法将被用来确定子宫和 滋养层表面参与识别，粘附， 植入时的融合事件。 该项目的目标包括：（1） 表征兔子宫上皮细胞之间粘附相互作用 培养和滋养层囊泡制备，并确定 甾体激素与基质细胞的相互作用; 2）鉴定 使用滋养层囊泡-上皮的功能性粘附组分 系统测定，3）鉴定感受态细胞质膜多肽 子宫上皮和滋养层表面， 互补配体-受体相互作用的潜在相关性 植入，以及4）表征在植入物中识别的表面成分， 目标2和3作为子宫容受性标志物的潜力 和滋养层分化的研究 程序. 兔子宫容受性的几个标志物 已经确定，并在体外模型将采用提供了承诺， 确定这些材料的功能性粘合剂或融合作用， 识别另外的粘合剂膜组分。 结果 这将增加我们对潜在机制的理解。 一个接受性子宫的发展，区别于一个难治性子宫， 置入

The objective of the research proposed is to identify and characterize properties of the rabbit uterine surface which are required for adhesion and fusion with the trophoblastic surface of an implanting embryo. The hypothesis to be tested is that the epithelial surface is the primary site for mediation of hormonally-regulated receptiveness of the uterus, and that apical membranes of opposed trophoblast and uterine cells will adhere via receptor-ligand interactions. Biochemical and structural approaches will be employed to identify components of uterine and trophoblast surfaces which participate in the recognition, adhesion, and fusion events at implantation. Aims of the project include 1) to characterize adhesion interactions between rabbit uterine epithelial cell cultures and trophoblast vesicle preparations and define the effects of steroid hormones and stromal cells on the interaction, 2) to identify functional adhesion components using the trophoblast vesicle-epithelium system assay, 3) to identify plasma membrane polypeptides of receptive uterine epithelium and the trophoblast surface which demonstrate complementary ligand-receptor interaction of potential relevance to implantation, and 4) to characterize the surface components identified in aims 2 and 3 with respect to potential as markers of uterine receptivity and trophoblast differentiation using structural and immunolocalization procedures. Several markers of uterine receptivity in the rabbit have been identified and the in vitro model to be employed offers promise for determining functional adhesive or fusigenic roles for these and for identifying additional adhesive membrane components. The results obtained will add to our understanding of mechanisms underlying the development of a receptive uterus as distinguished from one refractory to implantation.