ARCHITECTURE OF CORTICAL VISUAL AREAS MT, V3, AND DM

本提案中的实验旨在了解 三个皮质区的解剖和功能组织 在灵长类动物的视觉皮层。 老年人大脑皮层MT区实验分为三组， 世界猴子。 在第一组中，我们制作了长微电极 通过MT区的穿孔，记录单个单元的活动， 对一系列视觉刺激的反应。 视觉刺激都是 旨在揭示感受野的差异。 后来那些 差异可以与组织学标记相关， MT“条带”面积（用脱氧葡萄糖标记， 刺激）或MT“斑块”区域（用细胞色素标记 氧化酶）。 在第二组中，我们使用新的双标记脱氧葡萄糖协议 研究区域MT的功能结构。 一 实验比较了带与片的结构。 另外两个双标记脱氧葡萄糖实验将在 清醒的猴子 这些实验旨在 检查高级皮层视觉的功能组织 地区 在第三组中，我们将各种神经示踪剂注入视觉 大脑的哪些区域（或哪些列）， 区域）与MT频带或MT贴片共享连接。 最后一组实验是为了开始研究 V3和DM。 在一个实验中，我们计划从细胞中记录， 区域DM“条纹”和“条纹间”区域，以查看这些细胞 回答。 在另一种情况下，我们使用脱氧葡萄糖和细胞色素氧化酶， 染色技术，以研究功能组织， V3区。 最后，我们使用神经示踪剂来尝试发现 与V3补丁和interpatch的特定连接。

The experiments in this proposal are directed towards understanding the anatomical and functional organization of three cortical areas in primate visual cortex. There are three sets of experiments in cortical area MT of the Old World monkey. In the first set, we make long microelectrode perforations through area MT, recording single unit activity in response to a range of visual stimuli. The visual stimuli are all designed to reveal differences in receptive fields. Later those differences can be correlated with histological labelling of the area MT "bands" (labelled with deoxyglucose and specific visual stimuli) or the area MT "patches" (labelled with cytochrome oxidase). In the second set, we use a new double-label deoxyglucose protocol to investigate the functional architecture of area MT. One experiment compares the architecture of the bands to the patches. Two other double-labelled deoxyglucose experiments will be done in the awake behaving monkey. These experiments are designed to examine functional organization in higher-tier cortical visual areas. In the third set, we inject various neural tracers into visual areas of the brain, to find which areas (or which columns in which areas) share connections with the MT bands or the MT patches. The final set of experiments is designed to begin studying areas V3 and DM. In one experiment, we plan to record from cells in the area DM "stripes" and "interstripe" regions, to see how those cells respond. In another, we use deoxyglucose and cytochrome oxidase staining techniques to investigate the functional organization of area V3. Finally, we use neural tracers to try to discover the specific connections with the V3 patches and interpatches.