Single cell glycomics: mapping the surface glycan signature of cells

该项目旨在开发一种平台技术，用于单个细胞表面的多路复用糖链图谱，以通过利用一种概念上的新方法来应对功能糖组分的挑战。通过将新设计的血浆纳米颗粒与表面增强拉曼散射标签和多个特定的碳水化合物识别凝集素相结合，该项目预计将产生一种通用技术，能够对处于天然状态的单个细胞的表面多糖签名进行非破坏性条形码编码，并对代谢扰动做出反应。预期的项目成果包括通过能够容易地监测单细胞表面的变化来促进纳米生物技术、糖生物学和细胞生物学方面的知识。

This project aims to develop a platform technology for multiplexed glycan mapping of the surface of a single cell to address challenges of functional glycomics by utilising a conceptually new approach. By combining newly designed plasmonic nanoparticles with surface-enhanced Raman scattering tags and multiple specific carbohydrate-recognising lectins, this project expects to produce a generic technology that is capable of non-destructive barcoding of the surface glycan signature of single cells in their native state and in response to metabolic perturbations. Expected project outcomes include advancing knowledge in nanobiotechnology, glycobiology and cell biology by being able to easily monitor changes to the surface of single cells.