ANIMAL FACILITY IMPROVEMENT

目前，有60个个人研究项目需要使用 索尔克研究所的30名研究人员负责实验动物。 这些项目每年的直接费用总计7,913,459美元，包括 癌症研究中的分子、发育和系统研究 神经生物学；内分泌学；发育生物学和免疫学。在……里面 1988年，研究所发起了一项全面的努力，以更好地 支持现有和计划的动物研究项目的需求，以 更好地遵守《动物福利法》和PHS政策的规定 与实验动物护理和使用有关，并使研究所能够 成为AAALAC认证机构。在过去的三年里， 在动物设施和动物方面都有了改进 兽医护理和畜牧业项目以实现这些目标。 然而，需要小灵通的支持，以满足以下需要 癌症研究动物设施(CRAF)的改建和翻新 和设备采购，以实现研究所的总体目标 动物研究项目取得成果。 1.确保有效和常规的消毒和/或消毒 安装一个新的笼子和架子洗衣机，一个新的笼子和瓶子洗涤器， 一个新的地板式高压灭菌器和一个新的氯气冲洗站将进行升级 现有的洗车区；安装新的保险杠以保护 裸露的墙面；以及购买新的便携式不锈钢机架 用来关老鼠的笼子。 2.改善空气质素，尽量减少交叉污染 新的主排风道和新的排气阻尼器，调节风扇的螺距 叶片和重新平衡的空气供应和排气，以实现可接受的 房间空气换气量/小时和正确的气流模式。 3.建立一个符合NIH标准的集中管理的手术区，并 通过提供人员配置和配备中央 位于大鼠、猫和猴子的主要生存手术的手术区。 4.最大限度地减少将病原体引入已建立的 通过提供一个有两个动物隔间的隔离区和 1个经过HEPA过滤的生物安全柜，与之相邻，但 与其他动物房在物理上分开；并购买五个 额外的HEPA过滤层流工作台，以确保维护 转基因和免疫缺陷小鼠的病毒抗体无状态 (辐射嵌合体、SCID和Nu/Nu)更换灭菌微隔离器时 笼子和进行小的实验程序。 5.满足美国国立卫生研究院和美国农业部关于猫和猴子的安置标准，并提高 通过用新的笼子取代现有的笼子来改善他们的心理健康 不锈钢笼子。

Presently, 60 individual research projects which require the use of laboratory animals are conducted by 30 investigators at The Salk Institute. These projects, with annual direct costs totalling $7,913,459, include studies in cancer research; molecular, developmental, and systems neurobiology; endocrinology; developmental biology and immunology. In 1988, a comprehensive effort was initiated by the Institute to better support the needs of existing and projected animal research projects, to better comply with provisions of the Animal Welfare Act and PHS policies related to laboratory animal care and use, and to enable the Institute to become AAALAC accredited. Over the past three years, significant improvements have been made in both the animal facilities and the veterinary care and animal husbandry programs to achieve these objectives. PHS support will be needed, however, for the following necessary alterations and renovations of the Cancer Research Animal Facility (CRAF) and equipment purchases to bring the overall objectives for the Institute's animal research programs to fruition. 1. Ensure effective and routine sanitization and/or sterilization by installing one new cage and rack washer, one new cage and bottle washer, one new floor loading autoclave and one new chlori-flush station to upgrade the existing cagewashing area; installing new bumper guards to protect exposed wall surfaces; and purchasing new portable stainless steel racks for holding rat and mouse cages. 2. Improve air quality and minimize cross contamination by installing a new main exhaust duct and new exhaust dampers, adjusting the pitch of fan blades and rebalancing the air supply and exhaust to achieve an acceptable number of room air changes/hour and correct air flow patterns. 3. Establish a centrally managed surgery area that meets NIH standards and the needs of investigators by providing staffing and equipping a centrally located surgery area for major survival surgery on rats, cats and monkeys. 4. Minimize the potential for introducing pathogens into established animal colonies by providing a quarantine area with two animal cubicles and 1 HEPA-filtered biological safety cabinet that is adjacent to, but physically separate from, other animal housing rooms; and purchasing five additional HEPA-filtered laminar flow workbenches to ensure maintenance of the Viral-Antibody-Free status of transgenic and immunodeficient mice (radiation chimeras, SCID and nu/nu) when changing sterilized microisolator cages and performing minor experimental procedures. 5. Meet NIH and USDA standards for housing cats and monkeys and enhance their psychological well-being by replacing existing caging with new stainless steel cages.