AUTORADIOGRAPHIC/MRI IMAGING OF CEREBRAL FUNCTION

此应用程序的总体目的是改进现有的 技术和开发新的生化模型、方法和 放射自显影和磁共振实验技术 脑血流和代谢的成像及其临床意义 相互关系。 一种高性能、低成本成像系统的开发 用于定量分析和三维处理， 将完成放射自显影图像的显示。系统 使用新开发的内存密集型、基于查找表 算法实现了非常快速有效的处理速度 个人电脑。该系统将用于三维 脑血流的放射自显影定量分析及临床应用 动物体内的代谢示踪剂。 双标记和三标记放射自显影技术将是 为并行和顺序示踪研究而开发。使用这些 技术，各种代谢示踪剂的动力学将是 比较一下。这些研究的结果将用于评估 现有的动力学模型，并评估其意义和 他们的基本假设的局限性。在这个基础上，新的方法 以评估大脑的糖酵解和氧化 发展起来的。代谢研究随后将与研究相结合。 正常和异常情况下的血流量。 基于放射自显影研究，相应的磁共振 (MR)成像和光谱研究将在人体内进行 评价脑血流量与脑血流的关系 (脱氧血红蛋白位移成像)和大脑代谢(乳酸 光谱学)。 对大脑代谢和血流的基本认识 由这些研究提供的资料，将有助加深对 脑在正常和病理状态下的功能 动物模型(放射自显影)和人类模型(MR)。

The overall purpose of this application is to refine existing techniques and to develop new biochemical models, methods, and experimental techniques for autoradiographic and magnetic resonance imaging of cerebral blood flow and metabolism and their interrelationships. The development of a very high performance, low cost imaging system for quantitative analysis and three dimensional processing and display of autoradiographic images will be completed. The system uses newly-developed memory-intensive, lookup table-based algorithms to achieve very rapid effective processing speeds on personal computers. The system will be used for three dimensional quantitative analysis of autoradiograms of cerebral blood flow and metabolism tracers in animals. Double and triple label autoradiographic techniques will be developed for concurrent and sequential tracer studies. Using these techniques, the kinetics of various metabolic tracers will be compared. Results of these studies will be used to evaluate existing kinetic models, and to assess the significance and limitations of their basic assumptions. From this base, new methods for estimatiOn of cerebral glycolysis and oxidation will be developed. The metabolic studies will then be combined with studies of blood flow under normal and abnormal conditions. Based on autoradiographic studies, corresponding magnetic resonance (MR) imaging and spectroscopic studies will be performed In humans to evaluate the relationships between cerebral blood flow (deoxyhemoglobin-shift imaging) and cerebral metabolism (lactate spectroscopy). The basic understanding of cerebral metabolism and blood flow provided by these studies will promote greater understanding of the function of the brain in normal and pathological conditions in animal models (autoradiography) and in humans (MR).