SYNAPTOLOGY OF NEURONS INVOLVED IN REPRODUCTION

我们的目标是进一步阐明其解剖学和神经化学基础。 促性腺激素分泌。促黄体生成素释放激素 产生神经元在促性腺激素的分泌中起主要作用。 然而，LHRH神经元不包含类固醇受体，因此， 它们不是性腺激素的直接靶子。的反馈效应 这些激素对LHRH释放的影响主要是通过雌激素敏感性介导的。 与LHRH神经元相连的神经元和电路。 这份提案中概述的实验将进一步确定地点 这些传入系统的起源细胞，包括儿茶酚胺 和GABA能神经元及其相互和传入联系 与其他神经肽Y(NPY)和β-内啡肽(β-END)一起 已知在LHRH调节中发挥重要作用的神经元 神经元。这些联系的描绘将扩大我们的 对促性腺激素释放的中枢调节的认识 并解释为什么在不同的内分泌阶段，相同的递质 会对促性腺激素的释放产生相反的影响。此外，他们还会 有助于阐明内分泌疾病和年龄相关疾病的发病机制 卵巢周期功能障碍。“经典”关联的光和电子 显微技术将与最先进的电子技术相结合 显微双重免疫染色方法与正反向示踪剂 技巧。拟议的实验将定义： 1)雌激素参与的解剖学基础是什么- 敏感带是否参与调节LHRH的释放？ 2)哪种类型的儿茶酚胺细胞(S)参与了β-END- 介导的LHRH释放控制？ 3)涉及GABA能系统的位置和类型是什么 促性腺激素释放的直接和间接控制？ 4)哪些类型的神经肽Y神经元(S)参与了直接和间接 LHRH神经元的调节？ 5)β-端和β-端之间相互作用的突触基础是什么？ 多巴胺和脑干去甲肾上腺素系统？

Our goal is to further elucidate the anatomical and neurochemical basis of gonadotropin secretion. Luteinizing hormone releasing hormone (LHRH) producing neurons play the primary role in gonadotropin secretion. However, LHRH neurons do not contain steroid receptors and, therefore, they are not direct targets of gonadal hormones. The feed-back effects of these hormones on LHRH release are mainly mediated by estrogen-sensitive neurons and circuits which are connected to the LHRH neurons. Experiments outlined in this proposal will further determine the location of the cells of origin of these afferent systems, including catecholamine and GABAergic neurons, as well as their inter- and afferent connections with other neuropeptide Y (NPY)- and beta-endorphin (beta-END)-containing neurons, which are known to play important roles in the regulation of LHRH neurons. The delineation of these connections will extend our understanding of the central regulation of gonadotropin hormone release and elucidate why, in different endocrine stages, the same transmitters can exert opposite effects on gonadotropin release. Furthermore, they will help to elucidate pathomechanisms of endocrine disease-and age-related malfunctions of the ovarian cycle. "Classic" correlated light and electron microscopic techniques will be combined with state-of-the-art electron microscopic double immunostaining methods and antero-and retrograde tracer techniques. The proposed experiments will define: 1) What is the anatomical basis of the involvement of the estrogen- sensitive zona incerta in the regulation of LHRH release? 2) Which type(s) of catecholamine cells are involved in the beta-END- mediated control of LHRH release? 3) What are the locations and types of GABAergic systems involved in the direct and indirect control of gonadotropin release? 4) Which type(s) of NPY neurons are involved in the direct and indirect regulation of LHRH neurons? 5) What is the synaptological basis of interactions between beta-END and dopamine, and brain stem norepinephrine systems?