FOLDING RNA--FROM AIDS TO THE HUMAN GENOME

这项研究的最终目的是描述力量的特征 指导RNA折叠，并利用这一知识可靠地预测 从它们的序列中分析RNA分子的结构。因为大多数疾病都是 通过RNA，而一些病毒，包括艾滋病，是RNA， 结果可以指导合理的药物设计。结果也应该是 进一步解释由人类基因组决定的序列 项目。 研究将为结构预测提供基础 寡核苷酸的热力学和结构性质。我们的 对RNA折叠原理的理解将通过以下方式进行测试 比较预测的和已知的二级结构。关于基质的研究 I族核酶的结合和动力学将开始提供洞察力 转化为对RNA折叠很重要的三级相互作用。

The ultimate goal of this research is to characterize the forces directing RNA folding, and to use this knowledge to reliably predict structures of RNA molecules from their sequences. Since most diseases are mediated through RNA, and some viruses, including AIDS, are RNA, the results can lead to rational design of drugs. The results should also further the interpretation of sequences determined by the Human Genome Project. The foundation for structure prediction will be provided by studies of the thermodynamic and structural properties of oligonucleotides. Our understanding of the principles governing RNA folding will be tested by comparing predicted and known secondary structures. Studies of substrate binding and dynamics on a group I ribozyme will begin to provide insight into tertiary interactions important for RNA folding.