GENETICS OF TRANSOVARIOLE TRANSMISSION

人媒病毒性疾病仍然是非常重要的发病原因 和死亡率之间的关系。 许多虫媒病毒在地方性流行周期中通过 这种现象被称为跨卵巢传播（TOT）。 TOT指的是 虫媒病毒从受感染的雌性传播给其后代， 从而允许病毒在脊椎动物宿主外维持。 TOT在温带地区至关重要，因为它允许跨季节 虫媒病毒在滞育中越冬的维持 无脊椎动物宿主 交叉脑炎（LAC）病毒（布尼亚病毒科）是 美国小儿病毒性脑炎最重要的原因 States.通过其载体林地树洞蚊对LAC病毒的TOT 三列伊蚊是TOT系统中最具特征的系统之一， 成为研究TOT生物学的模型系统。 然而，实际上， 目前对Ae中TOT的遗传基础还一无所知。triseriatus或LAC。 这项建议的主要目标是量化的遗传基础， LAC病毒在Ae. triseriatus和映射涉及的载体基因。 然后将使用标记辅助选择（MAS）来选择载体菌株 在所有TO相关基因座上都是纯合的 MAS TOT耐药菌株 将用于识别TOT的解剖障碍，而MAS TOT 敏感菌株将用于评估遗传变异的作用， 不同LAC分离株在TOT中的作用。

Anthropod-borne viral diseases remain very significant causes of morbidity and mortality in humans. Many arboviruses in enzootic cycles through a phenomenon known as Transovarial Transmission (TOT). TOT refers to the transmission of the arbovirus from an infected female to her progeny and thus allows for maintenance of the virus outside of the vertebrate host. TOT is critical in temperate regions because it allows trans-seasonal maintenance of the arbovirus through the winter in its diapausing invertebrate host. The Crosse Encephalitis (LAC) virus (Bunyaviridae) is the most important cause of pediatric viral encephalitis in the United States. TOT of LAC virus by its vector, the woodland treehole mosquito Aedes triseriatus, is one of the best characterized TOT systems and has become the model system for studying TOT biology. However, virtually nothing is known about the genetic basis for TOT in Ae. triseriatus or LAC. The principal goals of this proposal are to quantify the genetic basis of TOT of LAC virus in Ae. triseriatus and map the vector genes involved. Marker-assisted selection (MAS) will then be used to select vector strains that are homozygous at all TO-relevant loci. MAS TOT refractory strains will be used to identify anatomical barriers to TOT while MAS TOT susceptible strains will be used to assess the role the genetic variation in different LAC isolates plays in TOT.