MOLECULAR BASIS OF PATHOGEN INDUCED CELL DEATH IN PLANTS

描述：这项建议侧重于监管和监管机制 执行病原体触发的细胞死亡程序，称为 植物的超敏反应(HR)。人力资源是快速发展的一部分 部署防御响应，其功能是限制病毒的传播 病原体感染。在此之前，制定了一项战略来研究 人力资源控制机制。调查者分离出了拟南芥植物 由于单基因突变，它们能够绕过病原体 暴露以激活HR和额外的防御系统。窝藏着植物 这些突变(被称为加速细胞死亡的ACD2)显示了HR， 但不含感染剂。因为ACD2突变是 隐性，研究者的模型是ACD2负性调节 HR和多种防御功能。了解规定，并 执行人力资源，这个项目的具体目标是：克隆 并对ACD2基因及其产物进行鉴定，以确定可能的 ACD2调控下的细胞死亡诱导和调控基因 确定在细胞死亡过程中与ACD2相互作用的其他基因 调控途径。最近在发病机制领域的研究表明 某些植物和动物病原体具有共同的致病基因。 在宿主一侧，植物和动物细胞都可以经历程序化 病原体攻击过程中的细胞死亡。有可能存在类似的情况 两者都使用细胞死亡的调节和执行机制 植物和动物。从事遗传学、分子生物学和 病原菌和寄主的生物化学使寄主的研究-- 利用拟南芥模型系统研究病原菌之间的相互作用 假单胞菌对阐明细菌感染的机制和作用具有吸引力 病原体诱导的细胞死亡。 除了激活HR外，ACD2突变体还激活了至少两个 通常只有在某些情况下才会触发的其他防御途径 病原体侵染野生型植物。这些路径需要两个信号： 已知的信号分子水杨酸和一种尚未表征的分子 信号。这项工作的长期目标是了解这些 通路由ACD2控制的信号协调调节(S)， 细胞死亡程序是如何被激活的，以及细胞死亡程序的组件是如何 信号转导通路(S)激活ACD2控制的防御。

DESCRIPTION: This proposal focuses on the mechanism of regulation and execution of a pathogen-triggered cell death program called the hypersensitive response (HR) in plants. The HR is part of a rapidly deployed defense response which functions to limit the spread of a pathogenic infection. Previously, a strategy was developed to study mechanism of HR control. The investigator isolated Arabidopsis plants which, due to single gene mutations, are able to bypass pathogen exposure to activate the HR and additional defenses. Plants harboring these mutations (called acd2 for accelerated cell death) show the HR, but harbor no infections agents. Since the acd2 mutations are recessive, the investigator's model is that ACD2 negatively regulates the HR and multiple defense functions. To understand the regulation and execution of the HR, the specific goals of this project are: to clone and characterize the ACD2 gene and its product, to identify the likely cell death inducing and regulatory genes under ACD2 control, and to identify additional genes that interact with ACD2 in the cell death regulatory pathway. Recent studies in the field of pathogenesis suggest that certain plant and animal pathogens have common pathogenicity genes. On the host side, both plant and animal cells can undergo programmed cell death during pathogen attack. The possibility exists that similar mechanisms of regulation and execution of cell death are used by both plants and animals. The ability to do genetics, molecular biology and biochemistry with both the pathogen and the host makes the study of host- pathogen interactions using the model system of Arabidopsis and Pseudomonas attractive for elucidating the mechanism and role of pathogen-induced cell death. In addition to activating the HR, the acd2 mutants activate at least two other defense pathways that are usually triggered only after certain pathogens infect wild-type plants. These pathways require two signals: the known signal molecule salicylic acid and an as yet uncharacterized signal. The long term goals of this work are to understand how these pathways are coordinately regulated by the ACD2-controlled signal(s), how the cell death program is activated and how the components of the signal transduction pathway(s) activate ACD2-controlled defenses.