B-LACTAMASE AND DEVELOPMENT OF MYXOCOCCUS XANTHUS

这个研究项目的目标是描述诱导因子的作用 β-内酰胺酶系统在黄色粘球菌发育程序中的作用。 最近的证据表明，M.xanthus将经历饥饿- 独立的孢子形成伴随着染色体的诱导 编码的β-内酰胺酶（ampC）。诱导型β-内酰胺酶的组分 肠杆菌中的细胞壁系统已经被证明是细胞壁所必需的。 回收利用。由于M. xanthus需要细胞壁 重塑，这导致了一个假设，即诱导的成分， β-内酰胺酶系统是正常发育所必需的。以下 将采取以下方法来检验这一假设：1）识别基因 通过PCR和转座子诱变参与β-内酰胺酶诱导，2） 通过监测这些基因在发育过程中的调节， 操纵子融合体的表达，和3）生化分析的作用， 基因产物在胞壁蛋白更新后细胞壁重塑中的作用 各种突变体背景中的成分。

The goal of this research project is to describe the role of the inducible beta-lactamase system in the developmental program of Myxococcus xanthus. Recent evidence indicates that M.xanthus will undergo starvation- independent sporulation with concomitant induction of a chromosomally encoded beta-lactamase (ampC). Components of the inducible beta-lactamase system in the enterobacteria have been shown to be required for cell wall recycling. Because sporulation in M. xanthus requires cell wall remodeling, this leads to the hypothesis that components of the inducible beta-lactamase system are required for normal development. The following approach will be taken to test this hypothesis: 1) identify the genes involved in beta-lactamase induction by PCR and transposon mutagenesis, 2) examine regulation of these genes during development by monitoring expression of operon fusions, and 3) analyze biochemically the role of gene products in cell wall remodeling by following turnover of murein components in various mutant backgrounds.