ELECTROSTATIC SAMPLING OF AIRBORNE MICROORGANISMS

描述：(改编自调查人员摘要)暴露于 农业、工业、室内和保健中的空气微生物 环境导致每年超过1亿次呼吸道疾病发作 美国的感染情况。由不同的商业机构进行并行监控 可用的生物气溶胶采样器不会对给定的 暴露水平，因为生物体的生存能力受到 他们在从空气中收集的过程中所暴露的压力 环境。这项研究的总体目标是使用静电 收集空气中微生物的机制，从而收集 生物体比目前通过惯性力实现的更温和 常规冲击器和冲击器。收集介质将是 移动围绕收集到的有机体的液体，并将它们移出 电场，从而将因电场而造成的生存损失降至最低 并在取样期间避免干燥。要找到 以最少的捕收量实现高效捕集的最佳条件 一种无臭氧感应充电的生物气溶胶发生器 产品将被设计、建造和使用，以研究不同 在收集过程中对生物体的电荷水平。电磁场中的电场 静电除尘器将由高变正 千伏/厘米电平恢复到其相当的负值。结果将是 相对于嵌入的自然存在的电荷水平进行评估 有机体。静电除尘板的角度将与 水平的、连续的流过下盘的流体将 将生物体从电场中移入液体池中，在那里 生物体被过滤掉，而留在液体中。新型取样器 将在实验室和农业环境中进行测试 目前存在高浓度的生物气溶胶。比较抽样将是 在常规冲击器和常规冲击器上执行。 分析了如何在室内和室内使用这种新的采集方法 生物气溶胶浓度低的卫生保健环境。

DESCRIPTION: (Adapted from the Investigator's Abstract) Exposure to airborne microorganisms in agricultural, industrial, indoor and health-care environments causes over 100 million episodes per year of respiratory infections in the US. Side-by-side monitoring by different commercially available bioaerosols samplers does not result in the same data for a given exposure level because the viability of the organisms is affected by the stresses they are exposed to during the process of collection from the air environment. The overall objective of this research is to use electrostatic mechanisms to collect the airborne microorganisms, thus collecting the organisms more gently than is currently achievable by the inertial forces in conventional impactors and impingers. The collection medium will be a moving fluid that surrounds the collected organisms and moves them out of the electric field, thus minimizing losses in viability due to the electric field and avoiding desiccation during the sampling period. To find the optimal conditions for high efficiency collection with the least amount of viability loss, a bioaerosol generator with induction charging and no ozone production will be designed, built and used to study the effect of different charge levels on the organisms during collection. The electric field in the electrostatic precipitation unit will be varied from a high positive kilovolt/cm level to its equivalent negative value. The results will be evaluated relative to the naturally present charge levels embedded in the organisms. The electrostatic precipitation plates will be at an angle to the horizontal, and a continuous flow of fluid over the lower plate will remove the organisms from the electric field into a liquid pool where the organisms are filtered out while remaining in the liquid. The new sampler will be tested in the laboratory and in an agricultural environment with high concentrations of bioaerosols present. Comparison sampling will be performed against a conventional impactor and a conventional impinger. Analysis will be made on how to use the new collection method in indoor and health-care environments where the bioaerosol concentration is low.