PROTECTIVE IMMUNITY IN MICE TO PNEUMOCOCCAL POLYSACCHARIDE PROTEIN

关于产妇免疫和化学制剂影响的研究仍在继续 在环境中对肺炎链球菌多糖的免疫应答 (PSS)与灭活肺溶血素(PLY)结合。各种共轭 尝试了还原胺化和PS衍生化等技术 目的：提高PS抗原的稳定性和免疫原性。年青的 来自母亲的小鼠，用9V PS免疫，灭活 溶气素(PLY)或9V PS-PLY结合物，在妊娠期间接受 出生后额外注射相同的抗原。剂量越大，诱导越高 未治疗与相同剂量的特异性IgM抗体应答的比较 控制。还进行了研究，以检查所造成的毒性影响 通过某些药物和生物制品与免疫的相互作用 系统。给怀孕14天的怀孕小鼠注射 醋酸可的松或灭虫剂，一种有机磷杀虫剂。之后 出生后，3周大的幼鼠暴露在吸入室中 使用相同的化学物质。后来，这些幼鼠被注射了9V型病毒 PS-PLY共轭化合物。在5周龄时，收集血清样本，并 正在测定9V免疫球蛋白和9V免疫球蛋白M抗体反应。这些研究 建议制定一项有效的免疫战略 肺炎球菌疫苗，并提供更好的理解可能 环境对结合免疫原性的影响。

Studies continued on effects of maternal immunization and chemical agents in the environment on the immune response to pneumococcal polysaccharides (PSs) conjugated to inactivated pneumolysin (ply). Various conjugation techniques including reductive amination and PS-derivatization were tried to improve the stability and immunogenicity of the PS antigen. Young mice from mothers, that were immunized with type 9V PS, inactivated pneumolysin (ply) or 9V PS-ply conjugate during gestation, received an additional dose of the same antigen after birth. The dose induced higher specific IgM antibody response compared to the same dose in non-treated controls. Studies were also conducted to examine the toxic effects caused by interactions of certain drugs and biological products with the immune system. Pregnant mice at 14 days of gestation were injected with cortisone acetate or Fenition, an organic phosphorus insecticide. After birth, young mice at 3 weeks of age were exposed in an inhalation chamber to the same chemicals. Later, the young mice were injected with type 9V PS-ply conjugate. At 5 weeks of age, serum samples were collected, and 9V IgG and 9V IgM antibody responses are being determined. These studies suggest the development of an effective strategy for immunization with pneumococcal vaccine, and provide a better understanding of possible environmental effects on conjugate immunogenicity.