POPULATION GENETICS OF AEDES AEGYPTI IN THE WEST INDIES

描述 这项申请的父母补助金的长期目标是确定， 分离和表征与易感性相关基因，或 蚊子物种对疟原虫寄生虫的抗性， 制定基于遗传学的疟疾控制战略 破坏蚊子媒介能力。 该项目的具体目标 是：1. 为了分离影响鸡疫霉易感性的基因， AE.埃及，2. 敲除已识别的基因，以确定它们是否 确实控制易感性，3. 以确定是否寄生虫暴露选择 对于等位基因的特定群体，以及4. 构建RFLP连锁图谱 冈比亚按蚊是人类疟疾的宿主。 这个FIRCA应用程序描述了直接扩展结果的实验 获得的母项目，研究种群动态的Ae。 埃及，特别是因为它涉及蚊子控制措施。 AE. 埃及是登革热病毒和黄热病病毒的宿主。 登革热 病毒是热带地区的一种流行病， 对抗这种疾病的方法是用杀虫剂消灭病媒， 这并不完全成功。 该项目将利用RFLP 由父项目生成的地图，以探索遗传多样性， 这些蚊子和蚊子的种群动态， 使用杀虫剂。 我们的目标是了解蚊子 杀虫剂应用后的生存，以制定更有效的 控制策略。 具体而言，特立尼达和托巴哥的四个地点已被选定， 分析. 鸡蛋将收集从四个网站之前，2-3周 施用杀虫剂后和施用杀虫剂后3-4个月。 来自所得个体蚊子的DNA将通过Southern杂交进行表征。 使用10-12个RFLP标记进行印迹，该标记由父母资助开发。 RFLP基因型的 将评价各个研究中心的频率， Hardy-Weinberg期望 为了评估遗传差异， 群体，遗传距离矩阵将使用Nei's 无偏遗传距离估计量 将对数据进行分析，以确定以下人员的遗传相似性水平 从不同的抽样地点的人口，并评估人口 杀虫剂处理后的动态 要问的问题是：1。 是 由于抗性种群的选择，杀虫剂后的存活率， 存活是不接触杀虫剂的函数吗？ 是 由于抗药性的扩大，使用杀虫剂后的种群增加 人口或来自其他地方的移民。

DESCRIPTION The long term goal of the parent grant for this application is to identify, isolate, and characterize genes associated with susceptibility or refractoriness of mosquito species to Plasmodium parasites and to use this information to develop a malaria disease control strategy based on genetic disruption of mosquito vector competence. The specific aims of this project are: 1. to isolate genes that influence P. gallinaceum susceptibility from Ae. aegypti, 2. to knock out the identified genes to determine if they indeed control susceptibility, 3. to determine if parasite exposure selects for specific populations of alleles, and 4. to construct a RFLP linkage map for the mosquito Anopheles gambiae, the host for the human malaria. This FIRCA application describes experiments that directly extend results obtained in the parent project to study the population dynamics of Ae. aegypti particularly as it relates to mosquito control measures. Ae. aegypti is the host for Dengue virus as well as yellow fever virus. Dengue virus is a prevalent infection in tropical areas and the only measures against this disease are vector elimination by insecticides, an approach that has not been entirely successful. This project will utilize the RFLP map generated by the parent project to explore the genetic diversity of these mosquitoes and the population dynamics of mosquitoes as a result of insecticide usage. The goal is to understand the mechanism for mosquito survival after insecticide application in order to develop more effective control strategies. Specifically four sites in Trinidad and Tobago have been selected for analysis. Eggs will be collected from the four sites before, 2-3 weeks after insecticide application, and 3-4 months after insecticide application. DNA from resulting individual mosquitoes will be characterized by Southern blots using 10-12 RFLP markers developed by the parent grant. RFLP genotype frequencies at individual study sites will be evaluated with respect to Hardy-Weinberg expectations. To assess genetic divergence between populations, the genetic distance matrices will be calculated using Nei's unbiased genetic distance estimator. The data will be analyzed to determine levels of genetic similarity among populations from different sample sites and to assess the population dynamics after insecticide treatment. Questions to be asked are: 1. is survival after insecticides due to the selection of resistant populations or is survival a function of non contact with insecticides, and 2. is the increase in population after insecticide due to expansion of a resistant population or due to immigration from other sites.