IMMUNODOMINANT TARGETS OF CMI TO CRYPTOSPORIDIUM

描述：（改编自申请人的摘要）。该第二 重新提交题为“CMI对隐孢子虫的免疫显性靶点”的资料 是鉴定免疫应答中重要的隐孢子虫抗原， 复苏申请人提出鉴定和分离重组C. 使用多克隆和单克隆方法从cDNA文库中获得细小病毒抗原 针对隐孢子虫蛋白产生的抗体，以评估 纯化的重组蛋白的免疫原性，使用T细胞 作为凝胶纯化蛋白或硝酸纤维素的增殖测定， 结合蛋白（T细胞免疫印迹测定），以确定是否 这些抗原引起T细胞应答，并最终分离T细胞 这些克隆能引起对这些抗原的强烈反应， 他们感染的SCID小鼠，以确定这些是否可以减少寄生虫 加载或诱导恢复。

DESCRIPTION: (Adapted From Applicant's Abstract). This second resubmission entitled "Immunodominant Targets of CMI to Cryptosporidium" is to identify important cryptosporidial antigens in immune response and recovery. The applicant proposes to identify and isolate recombinant C. parvum antigens from a cDNA library using polyclonal and monoclonal antibodies generated against cryptosporidial proteins, to evaluate purified recombinant proteins for their immunogenicity using a T cell proliferation assay as either gel purified protein or a nitrocellulose- bound protein (T cell immunoblot assay) in order to determine whether these antigens elicit T cell responses and finally to isolate T cell clones that elicit strong responses to these antigens and then engraft them to infected SCID mice to determine whether these can reduce parasite load or induce recovery.