CORE--EXPERIMENTAL IMMUNOLOGY FACILITY

实验免疫学核心设施将提供国家- 基础和临床CFAR使用的最先进的免疫学程序 调查人员。将提供免疫学研究的基本分析方法。 以服务收费为基础。费用结构将包括部分 来自核心补助金的补贴，这将使高质量的技术 可用于参与众多研究项目的人员。 其他目标包括培训来自其他国家的人员 实验室，并作为提供免疫试剂的资源 CFAR社区。长期目标是促进研究 并促进CFAR调查人员之间的合作。单个组件 这一核心基金包括：(A)分子免疫学。实验室 已经开发出用于分析细胞因子的独特专业知识和试剂， 非人灵长类动物中的趋化因子、趋化因子受体和生长因子 以及人类细胞和组织。(B)流式细胞术。新型流式细胞术 最近已经引入了检测特定细胞的方法- 对HIV的介导性免疫反应，包括抗原特异性标记 含可溶性MHC-四聚体的细胞及细胞内细胞因子的检测 由特定刺激的细胞产生。埃默里中心的目标是 对于艾滋病研究，流式细胞仪设施将提供这些新的 技术，以及传统的免疫表型分析，以临床 以及基础研究，调查感染艾滋病毒的人的特异性免疫反应 人类，并将其应用于用于病因学研究的灵长类动物模型 和疫苗的开发。(C)体液免疫反应的评估。这 实验室将提供标准化的分析方法，包括抗体- 捕获ELISA检测HIV/SIV抗GAG和抗环境抗体 抗体，CEM x 174共培养检测血清中和抗体 对各种HIV和SIV毒株的反应，以及Western blotting对 检查针对HIV或SIV的血清学反应的广度。(D)共焦 显微镜和免疫组织化学。共聚焦显微镜和 免疫组织化学设施将使CFAR成员获得高 分辨率，细胞的数字共聚焦图像。应用程序示例 包括病毒抗原在受感染人群中定位和共定位 含有病毒的亚细胞室的识别细胞 蛋白质，细胞蛋白质定位的影响的分析 不同的刺激(例如，当细胞 激活)，以及病毒核酸的定位。

The experimental Immunology Core Facility will provide expertise in state- of-the-art immunological procedures for used by basic and clinical CFAR investigators. Essential assays for immunology research will be provided on a fee-for-service basis. The fee structures will include a partial subsidy from the core grant, which will enable highly qualified technical personnel to be available to participate in numerous research projects. Additional objectives include training of personnel from other laboratories, and serving as a resource to provide immunological reagents to the CFAR community. The long term objectives are to facilitate research and promote collaboration among CFAR investigators. Individual components of this Core Facility include: (a) Molecular Immunology. The laboratory has developed unique expertise and reagents for analysis of cytokines, chemokines, chemokine receptors, and growth factors in non-human primates as well as human cells and tissues. (B) Flow cytometry. New flow cytometry methods have recently been introduced for detection of specific cell- mediated immune responses to HIV, including labeling of antigen-specific cells with soluble MHC-tetramers and detections of intracellular cytokines produced by specifically stimulated cells. The goal of the Emory Center for AIDS Research Flow Cytometry Facility will be to provide these new techniques, as well as traditional immunophenotyping analysis, to clinical and basic studies investigating HIV-specific immune responses in infected humans, and to apply them in primate models used for pathogenesis studies and vaccine development. (C) Evaluation of Humoral Immune Responses. This laboratory will provide standardized assays for analysis include antibody- capture ELISAs for the detection of HIV/SIV anti-gag and anti-env antibodies, CEM x 174 co-culture to detect serum neutralizing antibody responses against various HIV and SIV strains, and Western blots to examine the breadth of serologic response against HIV or SIV. (D) Confocal Microscopy and Immunohistochemistry. The confocal microscopy and immunohistochemistry facility will enable CFAR members to obtain high resolution, digital confocal images of cells. Examples of applications include localization and co-localization of viral antigens in infected cells, identification of subcellular compartments, containing viral proteins, assays of effects of localization of cellular proteins after different stimuli (e.g. capping of cell surface proteins when cells are activated), and localization of viral nucleic acids.