IGF-IIR, IMPRINTING, AND PALATE DEVELOPMENT

描述：调查人员已经确定了三个具体目标 解决IGF-IIR参与腭部发育和 因此，在腭部闭合。这些目标是：1)确定 单倍型特异率与腭裂发育的关系 IGF-IIR的mRNA和蛋白表达；2)显示母体效应 利用母本正反交表达IGF-IIR mRNA和蛋白， 以及3)确定正常的宫内环境是更大还是更大 IGF-IIR基因相似或不同对IGF-IIR基因的影响较小 关于发育变异。第一个目标将由 定量和定性检测IGF-IIR的mRNA和蛋白水平 在四天孕期内从同基因品系的小鼠的腭架上。 这些水平将通过腭部细胞增殖数据进行检查，以 确定这些端点之间是否存在关联。第二个目标 将是第一个目标下的实验的重复；然而， 用于这些实验的动物将是来自中国的(F1)杂交小鼠 同源小鼠品系的正反交。以这种方式， 调查人员可以确定这一因素是否与母亲的 已知的IGF-IIR基因是这样做的。最终目标将由一个 纯合子B10.A小鼠后代增殖细胞核抗原结果的统计分析 染色体同源部分杂合的小鼠后代 17，其中应存在四种基因型别。

DESCRIPTION: The investigators have identified three specific aims to address the hypothesis that IGF-IIR is involved in palatal growth and consequently in palatal closure. These aims are: 1) to determine the relationship between haplotype-specific rates of palatal development and IGF-IIR mRNA and protein expression; 2) to demonstrate a maternal effect on IGF-IIR mRNA and protein expression using matroclinus reciprocal crosses, and 3) to determine if the normal intrauterine environment is of greater or lesser influence than the similarity or dissimilarity of IGF-IIR genotype with respect to developmental variation. The first aim will be examined by quantitatively and qualitatively determining IGF-IIR mRNA and protein levels in palatal shelves from congenic strains of mice over four gestational days. These levels will be examined with palatal cell proliferation data to determine if there is a correlation between these endpoints. The second aim will be a repeat of the experiments under the first aim; however, the animals to be used for these experiments will be the (F1) hybrid mice from reciprocal crosses of the congenic mouse strains. In this manner the investigators can determine if the factor segregates with the maternal genotype as IGF-IIR is known to do. The final aim will be examined by a statistical analysis of PCNA results in offspring from homozygous B10.A mice vs offspring from mice heterozygous for the congenic portion of chromosome 17 in which four genotypes should be present.