IGF-IIR, IMPRINTING, AND PALATE DEVELOPMENT

IGF-IIR、印记和味觉发育

• 批准号: 2015393
• 负责人: Michael Melnick
• 金额: $ 4.04万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2015393
• 关键词: RNase protection assay; cell proliferation; cleft palate; developmental genetics; disease /disorder etiology; embryo /fetus cell /tissue; embryo /fetus toxicology; environmental toxicology; gene expression; genetic strain; genotype; growth factor receptors; haploidy; insulinlike growth factor; laboratory mouse; messenger RNA; northern blottings; polymerase chain reaction; receptor expression; western blottings

DESCRIPTION: The investigators have identified three specific aims to address the hypothesis that IGF-IIR is involved in palatal growth and consequently in palatal closure. These aims are: 1) to determine the relationship between haplotype-specific rates of palatal development and IGF-IIR mRNA and protein expression; 2) to demonstrate a maternal effect on IGF-IIR mRNA and protein expression using matroclinus reciprocal crosses, and 3) to determine if the normal intrauterine environment is of greater or lesser influence than the similarity or dissimilarity of IGF-IIR genotype with respect to developmental variation. The first aim will be examined by quantitatively and qualitatively determining IGF-IIR mRNA and protein levels in palatal shelves from congenic strains of mice over four gestational days. These levels will be examined with palatal cell proliferation data to determine if there is a correlation between these endpoints. The second aim will be a repeat of the experiments under the first aim; however, the animals to be used for these experiments will be the (F1) hybrid mice from reciprocal crosses of the congenic mouse strains. In this manner the investigators can determine if the factor segregates with the maternal genotype as IGF-IIR is known to do. The final aim will be examined by a statistical analysis of PCNA results in offspring from homozygous B10.A mice vs offspring from mice heterozygous for the congenic portion of chromosome 17 in which four genotypes should be present.

研究人员确定了三个具体目标， 解决IGF-IIR参与腭生长的假设， 从而影响腭闭合。 这些目标是：1）确定 单倍型特异性腭发育率与 IGF-IIR mRNA和蛋白表达; 2）证明母体对IGF-IIR mRNA和蛋白表达的影响。 使用matroclinus正反交的IGF-IIR mRNA和蛋白质表达， 和3）确定正常子宫内环境是否大于或等于 IGF-IIR基因型的相似性或不相似性的影响较小 关于发育变异。 第一个目标将由 定量和定性测定IGF-IIR mRNA和蛋白水平 在妊娠四天的同类系小鼠的腭架中。 这些水平将与腭细胞增殖数据进行检查， 确定这些端点之间是否存在相关性。 第二个目的 将是第一个目标下的实验重复;然而， 用于这些实验的动物将是（F1）杂交小鼠， 同种小鼠品系的相互杂交。 以这种方式 研究人员可以确定该因素是否与母体分离， IGF-IIR基因型是已知的。 最终目标将由 纯合子B10.A小鼠后代PCNA结果的统计分析 与来自染色体同源部分杂合子小鼠的后代相比， 17，其中四个基因型应该存在。