NOVEL METHOD FOR RAPID SEPARATION AND DETECTION OF DRUGS

描述：(申请人摘要) PI最近开发了一种超快速、高分辨率的方法来检测 滥用药物及相关化合物的分析测定。基于 离子迁移率分离电喷雾电离与质谱学 检测时，这种方法可以分离出分辨率相当的化合物 在毫秒时间尺度上与传统的层析方法相比， 检测下限与气相色谱后可能达到的下限类似。在……里面 色谱项，理论牌号大于20万有 在不到一秒的时间内测量，伴随的平板高度为600 nm 在检测下限小于1 ppb(ng/m L)。而离子迁移率 光谱分析已被常规用于街道检测挥发性物质 滥用药物，它已经不符合检测到的极性和 生物基质中的非挥发性药物。这项拨款申请提出了 评价电喷雾/离子迁移率/质谱学 (ESI/IMS/MS)作为快速、灵敏测量的分析方法 从复杂的基质中提取滥用药物并将其作为基础研究 阿片类药物和其他阿片肽的构象状态测量仪器 利息。该项目分为三个阶段，1)评估 ESI/IMS/MS作为定量分析的快速分离检测装置 和液体样品中滥用药物的定性检测；2) 二次电喷雾电离/IMS/MS作为一种检测方法的评价 从蒸气样品中测定滥用药物：和3)构象 目标多肽的表征和异构体分离。此外 对于独立的设备，仪器将同时耦合到液体和 气相色谱。该项目的成功完成不仅将 产生了一种分析方法，用于快速定量和 识别滥用药物，但也将提供一个研究工具 可直接与体内透析实验相结合或用于获得 高分辨率的时空药物浓度分布 细胞外空间。

DESCRIPTION: (Applicant's Abstract) The PI has recently developed an ultra-rapid, high-resolution method for the analytical determination of drugs of abuse and related compounds. Based on electrospray ionization with ion mobility separation and mass spectrometric detection, this method separates compounds with resolving powers comparable to traditional chromatographic methods on a millisecond time scale and with detection limits similar to that possible after gas chromatography. In chromatographic terms, theoretical plate numbers larger than 200,000 have been measured in less than a second with concomitant plate heights of 600 nm at detection limits less that 1 ppb (ng/mL). While ion mobility spectrometry has been routinely used for the street detection of volatile drugs of abuse, it has not been compatible with the detection of polar and non-volatile drugs in biological matrices. This grant application proposes to evaluate electrospray/ion mobility spectrometry/mass spectrometry (ESI/IMS/MS) as an analytical method for the rapid and sensitive measurement of drugs of abuse from complex matrixes and as a fundamental research instrument to measure conformational states of opioid and other peptides of interest. The project is divided into three phases, 1) the evaluation of ESI/IMS/MS as a rapid separation and detection device for the quantitative and qualitative determination of drugs of abuse from liquid samples; 2) the evaluation of secondary electrospray ionization/IMS/MS as a method for the determination of drugs of abuse from vapor samples: and 3) conformer characterization and isomer separation of peptides of interest. In addition to a stand-alone device the instrument will be coupled to both liquid and gas chromatography. Successful completion of the project will not only result in an analytical method for the rapid quantification and identification of drugs of abuse, but will also provide a research tool that can be coupled directly to in vivo dialysis experiments or used to obtain highly resolved spatial and temporal drug concentration profiles in extracellular space.