REGENERATION OF CD4 LYMPHOCYTES

描述（改编自申请人的摘要）：本申请的主要目的是 项目是确定是否（i）细胞凋亡的循环池，或（ii）细胞 周期停滞导致CD4细胞再生失败， (iii)识别与之相关的潜在细胞损伤。 朝向 为了实现这一目标，研究人员将重点关注纵向样本， 在抗病毒治疗的急性期 他们将通过流式细胞仪 分析细胞因子受体的存在或强度，粘附 分子、凋亡标志物、DNA含量和限制性酶切点相关 Ki67表达CD4细胞上的细胞周期蛋白及其离体细胞因子应答。 通过比较在显示不同的时间点获得的数据， CD4淋巴细胞再生的动力学，他们希望更好地定义 增强或抑制外周CD4扩增的病症。

DESCRIPTION (adapted from applicant's abstract): The primary aim of this project is to determine if (i) apoptosis of the cycling pool, or (ii) cell cycle arrest is contributing to the failure of CD4 cells to regenerate, and (iii) identify the potential cellular lesions associated therewith. Towards this goal, the investigators will focus on longitudinal samples obtained during the acute phase of anti-viral therapy. They will determine by FACS analysis the presence or intensity of cytokine receptors, adhesion molecules, apoptotic markers, DNA content, and restriction point-related cyclins on Ki67-expressing CD4 cells and their ex vivo cytokine response. By comparing the data obtained at time points which display different kinetics of CD4 lymphocyte regeneration, they hope to better define the conditions which potentiate or inhibit peripheral CD4 expansion.