3D TISSUE CHANGE DETECTION/QUANTIFICATION METHOD

长期目标是实现快速、灵敏和低成本 组织结构变化的检测和可靠的量化 由药物、毒素、荷尔蒙、疾病和 物理/化学刺激。这样的研究构成了药学的核心 研究、毒理学、人类和动物用产品安全测试 和有效性，以及大量的生物学基础研究 还有医学。在这些研究中，有必要进行比较研究 对两批组织进行细胞学检测-其中一批是正常的，另一批是 其他的则是由利益代理人转变的。当更改是 细微的(如癌症)，重要的是处理大量的 组织。目前，所有这些都是手动完成的，所以方法很慢， 繁琐，昂贵，缺乏定量的准确性和 敏感度。通常，重要的研究被放弃或没有尝试 这些原因。 所提出的研究将产生一种大规模、充分 自动化的组织水平变化检测和量化。它是基于 厚(20-500微米)、宽(1-10毫米)组织样品的三维显微镜 在一系列重叠窗口中，然后是自动3-D图像 分析。这将产生一个3-D细胞测量数据库， 通过统计方法进行分析，以检测和量化变化。 建议的商业应用： 由此产生的软件产品预计将是即时和长期的- 制药公司的长期利益，必须评估的公司 常见的日常用品(如肥皂、洗发水、化妆品、泻药、 药膏)的健康风险和有效性，国家卫生部门 (特别是毒理学)，大多数主要的研究型医院(特别是各部门 病理学)，以及世界各地主要大学的生物系。

The long-term objective is to enable rapid, sensitive, and low-cost detection, and reliable quantification of structural changes in tissue caused by agents such as drugs, toxins, hormones, diseases, and physical/chemical stimuli. Such studies form the core of pharmaceutical research, toxicology, human- and animal-used product testing for safety and effectiveness, as well as a large body of basic research in biology and medicine. In these studies, it is necessary to perform comparative cytometry on two batches of tissue - one of which is normal, and the other is transformed by the agent of interest. When the changes are subtle (e.g., cancer), it is important to process large amounts of tissue. Currently, all this is done manually, so the methods are slow, tedious, expensive, and lacking in quantitative accuracy and sensitivity. Often, important studies are abandoned or not attempted for these reasons. The proposed research will result in a method for large-scale, fully automated tissue-level change detection and quantification. It is based on 3-D microscopy of thick (20-500 microm), wide (1-10mm) tissue samples in a series of overlapping windows, followed by automated 3-D image analysis. This produces a database of 3-D cytometric measurements that are analyzed by statistical methods to detect and quantify changes. PROPOSED COMMERCIAL APPLICATION: The resulting software product is expected to be of immediate and long- term interest to pharmaceutical companies, companies that must evaluate common everyday products (e.g., soaps, shampoos, cosmetics, laxatives, ointments) for health risks and effectiveness, state health departments (esp. toxicology), most major research hospitals, (esp. departments of pathology), and biology departments of major universities worldwide.