CYTOKINE/CHEMOKINE ACTIONS ON DEVELOPING CNS NEURONS

描述(改编自申请者的摘要)：深入了解 在儿童神经艾滋病中观察到的认知和发育问题 研究人员建议检查几种细胞因子和 已报道在HIV-1感染者中枢神经系统中表达的趋化因子 病人。研究人员建议使用小脑浦肯野细胞培养 从胚胎啮齿类动物中检测重组细胞因子的作用 和趋化因子以三种不同的方式给药：急性、慢性和 戒烟。效果将通过形态分析来确定， 各种膜离子的免疫细胞化学、电生理记录 通道和神经递质受体特性，以及钙成像。 通过确定细胞因子/趋化因子在这些参数中的调节变化 开发神经系统，研究人员希望提供新的见解 这些因素在儿童中枢神经系统功能障碍中的潜在作用 神经艾滋病。

Description (adapted from applicant's abstract): To gain insights into the cognitive and developmental problems observed in pediatric neuroAIDS, the investigators propose to examine the effects of several cytokines and chemokines that are reported to be expressed in the CNS of HIV-1 infected patients. The investigators propose to use the cerebellar Purkinje cell culture system from embryonic rodents to determine the effects of recombinant cytokines and chemokines administered in three different modes; acute, chronic, and withdrawal. The effects will be determined by analysis of morphology, immunocytochemistry, electrophysiological recordings of various membrane ion channels and neurotransmitter receptor properties, as well as calcium imaging. By determining cytokine/chemokine-modulated changes in these parameters in developing neuronal system, the investigators wish to provide new insights into the potential roles of such factors in the CNS dysfunction in pediatric neuroAIDS.