CHEMICAL & GENETIC APPROACHES TO DEFINE CELL ACTIVATION

描述：两条研究路线，一条是研究引发 植物较高的细胞分裂和另一种与病程有关的 原核病原菌的这种植物，已交叉在单一的异种 生长因子参与了这两个事件的控制。结构分析 已经导致了一个激活的机制模型 事件。该模型提供了有价值的见解，使开发成为可能 可逆性和不可逆性农杆菌特异性抑制剂 根癌毒力基因的表达。通过利用抑制剂和 病原菌、特异性受体蛋白XPB的分子遗传学 蛋白质，已经被鉴定出来。概述的实验将(I)分离出 并对Xpa蛋白进行了鉴定，(Ii)鉴定了其作用机制 生长因子-XPB复合体激活双组分磷酸化 级联控制毒力基因表达，(Iii)筛选和鉴定 改变对生长因子敏感性的突变体，以及(Iv)分析 参与信号转导的自体激酶的特定突变体。 成功完成拟议中的实验将大大扩展 我们对原核生物的酚性感知的理解，至少提供了 一个模型，如果不是真核生物感知的蛋白质，以及提供 第一次洞察到双组件监管系统如何识别和 转换信号输出。相关增长因素在以下方面发挥关键作用 动植物系统及其机制的研究机会 在发病机制方面的因素是独一无二的。所获得的知识将 提供对真核细胞分裂的控制以及 定义发病机制中涉及的策略。两个实验室已经合并 他们的专业知识，以开发化学，生物化学和分子 有效执行这些任务所需的遗传方法 实验。

DESCRIPTION: Two lines of study, one dealing with factors that initiate higher plant cell division and the other involving pathogenesis in a prokaryotic pathogen of this plant, have intersected at a single xenognostic growth factor involved in the control of both events. Structural analysis of the growth factor has led to a mechanistic model for the activation event. This model has provided valuable insight and enabled the development of both reversible and irreversible specific inhibitors of Agrobacterium tumefaciens virulence gene expression. By exploiting the inhibitors and the molecular genetics of the pathogen, specific receptor proteins, Xpb proteins, have been identified. The outlined experiments will (i) isolate and characterize the Xpa proteins, (ii) characterize the mechanism by which the growth factor-Xpb complex activates a two-component phosphorylation cascade controlling virulence gene expression, (iii) select and characterize mutants that alter sensitivity to the growth factor, and (iv) analyze specific mutants of the autokinase involved in the signal transduction. Successful completion of the proposed experiments will significantly expand our understanding of phenolic perception of prokaryotes, providing at least a model, if not the proteins for eukaryotic perception, as well as provide the first insight into how two-component regulatory systems recognize and transduce signal output. Related growth factors serve critical roles in animal and plant systems and the opportunity to study the mechanism of these factors in the context of pathogenesis is unique. The knowledge gained will provide insight into the control of eukaryotic cell division as well as define strategies involved in pathogenesis. Two laboratories have combined their expertise in order to exploit the chemical, biochemical and molecular genetic approaches necessary for the efficient execution of these experiments.