MOLECULAR EPIDEMIOLOGY OF PERSISTENT HPV INFECTION

描述：这是一份竞争性的继续学习申请。 人乳头瘤病毒(HPV)生殖器持续存在于一大群Low 巴西圣保罗的收入妇女，这是宫颈癌的高危地区。这个 研究重点是致癌HPV类型与肿瘤发生发展的关系 宫颈上皮病变和癌。参与者经历了一系列 基于问卷的访谈，以获得性行为和其他潜在的 致病因素、宫颈涂片、聚合酶链式反应检测、人乳头瘤病毒血清学和 宫颈造影术。目标是 1研究持续性宫颈HPV的发病率和流行率 无症状的妇女； 2验证持续型HPV低增长高增长的假说 宫颈病变分级； 3检测HPV持久性的流行病学决定因素； 4寻找与宫颈病变相关的分子变异； 5为了确定HPV病毒载量是否与持久性有关， 以及低级别或高级别病变；以及 6研究抗体反应对HPV持久性和病变的作用 进步。 对于竞争更新，提出了另外两个目标： HPV持续感染和宫颈病变相关的7种单倍型HPV 损害；以及 8检测P53基因多态性是否对HPV持久性具有抵抗力 和病变的发展。

DESCRIPTION: This is a competing continuation application for continued study of human papilloma virus (HPV) genital persistent in a large cohort of low income women in Sao Paolo, Brazil, a high risk area for cervical carcinoma. The study focuses on the relationship of oncogenic HPV types on the development of cervical epithelial lesions and carcinoma. Participants undergo a series of questionnaire-based interviews to access sexual behavior and other potentially contributing factors, PAP smears, HPV testing by PCR, HPV serology and cervicography. The objectives are to 1 study incidence and prevalence of persistent cervical HPV in asymptomatic women; 2 verify the hypothesis that persistent HPV increases low and high grade cervical lesions; 3 detect epidemiological determinants of HPV persistence; 4 search for molecular variants associated with cervical lesions; 5 to determine whether HPV viral load is associated with persistence, and low or high grade lesions; and 6 study the role of antibody response to HPV persistence and lesion progression. For the competitive renewal, 2 additional aims are proposed: 7 identity HLA haplotypes associated with HPV persistence and cervical lesions; and 8 test whether p53 polymorphisms confer resistance to HPV persistence and lesion development.