MECHANISMS OF HYPERSENSITIVITY STATES

豚鼠是研究血管紧张素转换酶机制的良好模型。 即刻和迟发性超敏反应。在我们的实验室里，我们有 初步证据表明，我们可以使潜在的重要 嗜同型抗体、肥大细胞和 豚鼠过敏状态下的嗜碱性粒细胞。 到目前为止，我们的数据已经证明，IgG1和IgE都可以调节 豚鼠的肺收缩，它们似乎是通过 分离的组织受体。此外，我们已经能够分离出肺 组织肥大细胞，并有能力研究 这些抗体直接在这个细胞上发挥作用，已经被证明是 在即刻反应中起着重要作用。我们也有能力 分离纯种群的嗜碱性血液细菌(纯度为85-100%)。 基于这些观察，我们建议详细研究 IgG1和IgE肺受体的独特性质。我们计划做的是 这是通过悬浮从IgG1或IgE致敏的肺组织中获得的 在灌注室中同时测量动物的收缩和 在特定抗原刺激下释放的介质。我们也会 我想用嗜同型细胞抗体将这些组织研究扩展到 通过对肺肥大细胞中IgG1和IgE的研究来分离肺肥大细胞 受体和检测这些分离细胞的介质释放时 用IgG1或IgE抗体致敏。 我们建议的第二个独立但又相关的部分是研究 豚鼠皮肤离体粒的致炎作用 从纯化的嗜碱性血球中获得(纯度90-100%)。有了这个，我们 希望明确嗜碱性粒细胞参与迟发性痴呆的一些机制 含有嗜碱性细胞的皮肤反应。 就主要介体而言，豚鼠与人类的相似性 肥大细胞和嗜碱性粒细胞释放的物质使这些潜能的研究 在理解即时和延迟的类似事件中的重要性 人类的过敏症状态。

The guinea pig is an excellent model in which to study mechanisms of immediate and delayed hypersensitivity. In our laboratory, we have preliminary evidence suggestive that we can make potentially important observations as to the role of homocytotropic antibody, mast cells, and basophils in guinea pig hypersensitivity states. Our data to date has demonstrated that both IgG1 and IgE can mediate polmonary contraction in the guinea pig and they seem to do so through separate tissue receptors. Furthermore, we have been able to isolate lung tissue mast cells and have the capability to study the biochemistry of these antibodies directly on this cell which has been shown to play an important role in immediate reactions. We also have the capability of isolating pure populations of blood basophilis (85-100% purity). Based upon these observations, we propose to study in detail the distinctive nature of the IgG1 and IgE pulmonary receptors. We plan to do this by suspending pulmonary tissue obtained from IgG1 or IgE sensitized animals in a superfusion chamber and simultaneously measure contraction and mediators released upon stimulation with specific antigen. We also would like to extend these tissue studies with homocytotropic antibody to the isolated pulmonary mast cell by studying this cell for IgG1 and IgE receptors and examining mediator release from these isolated cells when sensitized with IgG1 or IgE antibodies. A second separate but yet related part of our proposal is to examine the inflammatory provoking potential in guinea pig skin of isolated granules obtained from purified blood baseophils (90-100% purity). With this we hope to define some of the mechanisms of basophil involvement in delayed cutaneous reactions containing basophils. The similarity of the guinea pig to humans as far as primary mediators released by mast cells and basophils makes these studies of potential importance in understanding similar events in immediate and delayed hypersensitivity states in humans.