MOLECULAR CORRELATES OF NEURONAL FUNCTION

我们提出了新的方法来建立分子相关的 哺乳动物背根神经节的功能和结构特征， 海马神经元 重点将放在a）这两个神经元上 使用多种突触介质的群体，和B）在突触介质中的方式， 哪些关键的分子决定因素被调节。 具体目标是：1） 确定初级传入神经元和海马神经元的分子特征 区分功能和/或形态亚类; 2） 确定肽和其他化学介质在细胞中所起的作用， 初级传入神经元和海马神经元的功能; 3）检查 调节膜受体的生化机制， 通道，以及这些事件与表达的可能耦合， 神经元特异性分子决定簇。 实验将在 在体内，在背根神经节和海马神经元的培养物中，以及在 海马切片。 建立形态学（光和电子 显微镜）和电生理学（微电极和膜片钳） 技术将与免疫细胞化学，生物化学， 药理学和分子遗传学方法。 一个设施， 产生特异性多克隆和单克隆抗体， 杂交组织化学将被四个共同使用 项目

We propose new approaches to establishing molecular correlates of functional and structural features of mammalian dorsal root ganglion and hippocampal neurons. The focus will be upon a) neurons of these two populations that employ multiple synaptic mediators, and b) the manner in which key molecular determinants are modulated. The specific aims are: 1) to determine molecular features of primary afferent and hippocampal neurons that distinguish between functional and/or morphological subclasses; 2) to determine the parts played by peptides and other chemical mediators in the function of primary afferent and hippocampal neurons; 3) to examine the biochemical mechanisms underlying modulation of membrane receptors and channels, and the possible coupling of such events to expression of neuron-specific molecular determinants. The experiments will be done in vivo, in cultures of the dorsal root ganglia and hippocampal neurons and in hippocampal slices. Established morphological (light and electron microscopic) and electrophysiological (microelectrode and patch clamp) techniques are to be coupled with immunocytochemical, biochemical, pharmacological and molecular genetic approaches. A facility for the generation of specific polyclonal and monoclonal antibodies and for in situ hybridization histochemistry will be set up for common use by the four projects.