A RESOURCE FOR ANALYSIS OF TWO-DIMENSIONAL PROTEIN GELS

建议建立一个生物技术资源设施， 二维凝胶 该设施将基于软件包 在冷泉港进行的2D凝胶分析 实验室 该软件将在VAX计算机系统上实现， 强大的图形和绘图功能。 用户将能够进行二维电泳下， 专业的2D凝胶实验室的标准化条件已经 建立在冷泉港实验室。 在凝胶被 准备和扫描的设施的工作人员，用户将有 可以根据需要随时访问专用图形工作站， 完成数据分析。 每个用户将能够比较来自 每一个新的样本都有他自己的文件和公众的数据， 数据库。 在分析过程中，用户可以制作幻灯片和照片 从图形显示器上显示的图像，以及凝胶的大彩色图， 可以绘制图案。 核心研究人员将提供计算机和图形 增强，它将进行研究活动，以保持和 增强大鼠、小鼠和人类细胞的蛋白质数据库。 该数据 将提供给设施的所有用户和科学家， 公众 将与 该设施概述了在以下领域的重大研究项目： 细胞转化、分化和小鼠发育。 服务 来自各种科学学科的用户也表达了强烈的 兴趣 该设施将培训其用户的计算机技术，它将 为希望了解更多信息的学生和科学家提供暑期课程 关于计算机分析的二维凝胶电泳。 计划 设施应该为我们的主要目标提供重大进展， 其目的是建立大的和普遍有用的蛋白质数据库， 在整个生物学领域更广泛地传播这项技术， 社区 从长远来看，数据库将提供更大的 了解成千上万的蛋白质可检测的二维 凝胶电泳，其中许多将是重要的诊断 疾病

A Biotechnology Resource facility is proposed for computer analysis of two-dimensional gels. The facility will be based on the software package for 2D gel analysis that has been written at the Cold Spring Harbor Laboratory. The software will be implemented on a VAX computer system with powerful graphics and plotting capabilities. Users will be able to have two-dimensional electrophoresis performed under standardized conditions in the specialized 2D gel laboratory already established at the Cold Spring Harbor Laboratory. After the gels are prepared and scanned by the staff of the facility, the user will have access to a dedicated graphics workstation for as long as needed to complete his data analysis. Each user will be able to compare data from each new sample with data from his own files and with the public databases. During his analysis, the user can make slides and photographs from images displayed on the graphic monitors, and large color maps of gel patterns can be plotted. The core research staff will provide the computer and graphics enhancements, and it will perform research activities to maintain and enhance the protein databases for rat, mouse, and human cells. This data will be available to all users of the facility and to the scientific public. The collaborative users who will interact heavily with the facility have outlined significant research projects in the fields of cellular transformation, differentiation, and mouse development. Service users from a variety of scientific disciplines have also expressed strong interest. The facility will train its users in the computer technology, and it will provide a summer course for students and scientists wanting to learn more about computer-analyzed two-dimensional gel electrophoresis. The planned facility should provide significant progress toward our major objectives, which are to establish large and generally useful protein databases and to disseminate the technology much more generally throughout the biological community. In the long range, the databases will provide greater understanding of the thousands of proteins detectable by two-dimensional gel electrophoresis, many of which will be important in the diagnosis of disease.