Visualising a bacterial stress response: gene product localisations movements and gene regulation at single molecule level in live bacterial cells

细菌是地球上最丰富的生命形式之一，对碳、氮、硫和磷的主要循环和转化做出了巨大贡献，这些循环和转化是功能性和健康的全球生活环境的特征。尽管在了解细菌细胞成分如何工作方面取得了巨大进展，但人们对它们在活细胞内的组织方式知之甚少，也不知道这些组织如何影响细胞的功能。使用一种使我们能够可视化活细胞内单个分子的方法，我们将以定量的方式观察细胞成分的动力学，这些成分使细胞在破坏其膜的压力下存活下来。实际上，我们将研究细胞的活动，并将能够描述细胞在其预应力状态以及在其适应状态。了解细胞成分是如何组织起来发挥功能的，这对于建立细胞生长和适应新环境的信号传导原理和相互作用网络是很重要的。

Bacteria comprise one of the most abundant life forms on the planet, and contribute hugely to, for example, the major carbon, nitrogen, sulphur and phosporus cycles and transformations that characterise a functional and healthy global living environment. Despite huge advances in understanding how the components of the bacterial cell work, little is known about how they are organised within the living cell, nor how such organisations impact upon the functionality of the cell. Using a method that enables us to visualise single molecules within living cells, we will look in a quantitative way at the dynamics of the cellular components that let the cell survive a stress that damages its membrane. In effect we will be studying the cell in action, and will be able to describe the cell in its pre-stressed state as well as in its adapted state. Gaining an appreciation and understanding of how the cells components are organised for function is important in establishing principles of signaling and the networks of interactions that allow cells to grow and adapt to new environments.