GENETIC DIFFERENTIATION IN THE AEDES ALBOPICTUS SUBGROUP

这项拟议研究的具体目标是分析基因 白纹伊蚊亚群6种与白纹伊蚊T0亚群的区别 从遗传学的角度描述了广布种A. 白纹伊蚊。白纹伊蚊亚群由11个亚群组成 其中白纹伊蚊是最重要的物种。它是一个载体， 东南亚的登革热和登革出血热。它很宽 分布范围从马达加斯加延伸到夏威夷。近年来，它已经 将其射程扩大到关岛，南太平洋，甚至在 田纳西州孟菲斯的灯光陷阱。令人惊讶的是，几乎没有人在 该亚群中该物种或其他相关物种的遗传学。建议数 研究将有助于弥合这一差距。 长期目标是利用A. 白纹伊蚊种群以及亚群中的某些相关物种 评估它们作为登革热有效媒介的潜力。 拟议的基因研究将提供必要的基础 登革热传播遗传学与登革热能力的未来分析 传播疾病的物种和菌株。 具体目的是刻画白纹伊蚊的遗传变异 以及白纹伊蚊亚群中的物种之间的 通过四种不同类型的分析。基因的间接测量 分歧将通过以下方式获得：1)与 收集的受精率、胚胎率和孵化率以及 杂交后代和回交后代的生存能力，以及2)利用 在一系列重复中的物种对，旨在产生 性隔离。将获得遗传分化的直接测量结果 来自染色体分化和电泳法的分析 酶的可变性。将通过以下方法检查染色体分化 一系列研究包括Giemsa C显带定位异染色质， 杂交种减数分裂分析及电泳法构建连锁图谱 比较以确定是否存在倒位、易位和 核DNA的缺失或复制以及细胞光度分析 内容。等位基因的UPGMA聚类和主成分分析 从等位酶研究中获得的频率数据将用于区分 将种群划分为子集。如果某些诊断等位基因、基因座或离散 等位基因频率差异或染色体变异存在于 登革热流行的白纹伊蚊地理株与 在非疫区，媒介能力可能会变得有效标记。

The specific aims of the proposed research are to analyze genetic differentiation between 6 species in the Aedes albopictus subgroup and to genetically characterize geographic variation in the widespread species, A. albopictus. The Aedes (Stegomyia) albopictus subgroup consists of 11 species of which A. albopictus is the most important. It is a vector of dengue fever and dengue hemorrhagic fever in southeast Asia. Its wide distribution extends from Madagascar to Hawaii. In recent years it has extended its range to Guam, the south Pacific and has even been reported in light traps in Memphis, TN. Surprisingly, little work has been done on the genetics of this or other related species in the subgroup. The proposed research will help bridge this gap. The long term objectives are to utilize the genetic characterization of A. albopictus populations as well as certain related species in the subgroup to evaluate their potential to serve as efficient vectors of dengue fever. The proposed genetic studies will provide the necessary foundation for future analyses on genetics of dengue transmission and competence of species and strains to transmit the disease. The specific aims, to characterize genetic variation within A. albopictus and between species in the albopictus subgroup, will be accomplished through four different types of analyses. Indirect measures of genetic divergence will be obtained through: 1) experimental hybridization with data collected on insemination, embryonation and egg hatch rates and on the viability of hybrid and backcross progeny, and 2) mate choice tests using species pairs in a series of replicates designed to produce indices of sexual isolation. Direct measures of genetic divergence will be obtained from analyses of chromosome differentiation and electrophoretic detection of enzyme variability. Chromosome differentiation will be examined through a series of studies including Giemsa C-banding to localize heterochromatin, meiotic analysis of hybrids and electrophoretically derived linkage map comparisons to determine the presence of inversions, translocations and deletions or duplications, and cytophotometric analysis of nuclear DNA content. UPGMA clustering and principal component analysis of allele frequency data obtained from allozyme studies will be used to differentiate populations into subsets. If certain diagnostic alleles, loci or discrete allelic frequency differences or chromosomal variants exist among geographic strains of A. albopictus from dengue epidemic versus non-epidemic areas, vector competence may become effectively marked.