MODIFICATION OF LOCAL AND SYSTEMIC ALLOGRAFT REJECTION

存在许多不同的机制来影响同种异体移植 拒绝。淋巴细胞在移植物中的作用是中心的 有充分证据表明，辅助性T细胞和 细胞溶解T细胞可影响移植物丢失。同样明显的是， 在移植物部位发生的局部事件与 对同种异体移植的全身反应。 海绵基质同种异体移植模型提供了通向细胞的便捷途径 聚集在同种异体移植排斥反应的部位。在……里面 与极限稀释分析相结合，可以识别 少量的致敏细胞，我们可以很容易地评估事件 与局部和全身同种异体移植免疫有关。使用这些 淋巴细胞而不是放射性标记的功能评估 不再需要将标记的细胞引入 血管内空间。我们计划使用这些技术来定义 这些局部和系统的事件根据组织 被移植了。一旦为各种组织定义了这些事件，我们 计划使用局部或全身免疫抑制来修改 他们。长期目标是利用局部免疫抑制来 使动物的系统免疫系统完好无损。

A number of different mechanisms exist to effect allograft rejection. The role of the lymphocyte is central in graft rejection; ample evidence exists that the helper T cell and the cytolytic T cell can effect graft loss. It is also clear that events occurring locally at the graft site are distinct from the systemic response to the allograft. The sponge matrix allograft model provides ready access to cells which accumulate at the site of allograft rejection. In combination with limiting dilution analysis which can identify small numbers of sensitized cells, we can readily assess events associated with local and systemic allograft immunity. Using these functional assessments of lymphocytes rather than radiolabel eliminates the need to introduce radiolabelled cells into the intravascular space. We plan to use these techniques to define these local and systemic events according to the tissue transplanted. Once these events are defined for various tissue we plan to use either local or systemic immunosuppression to modify them. The long term goal is to employ local immunosuppression to render the animal's systemic immune apparatus intact.