NUCLEIC ACID AND PROTEIN METABOLISM IN CARTILAGE

本研究建议的目的是为了继续研究正常 并特别注意病理上的关节软骨和骨骺软骨 在健康和各种疾病状态下组织的代谢物， 尤其是人类的骨性关节炎。将进行的研究包括 正常和病理组织中DNA复制活性的继续研究 软骨，特别强调使用流式细胞术；续 正常细胞和病理细胞合成产物和合成速率的定义 利用各种生化和新陈代谢技术的软骨 包括无细胞系统；对各种正常的 和代谢的病理控制机制(合成代谢和 分解代谢)正常和异常软骨的活动；寻找 疾病状态的细胞内和细胞膜标记物 病理性软骨，主要通过使用流式细胞仪；并继续 尝试分离、鉴定、提纯和测定 降解的最佳条件、底物、辅因子和缓蚀剂 正常和病变的关节软骨和骨骺软骨中的酶。

The purpose of this research proposal is to continue the study of normal and pathological articular and epiphyseal cartilage with special attention to the metabolims of the tissue in health and a variety of disease states, especially human osteoarthritis. The studies to be performed include a continued study of DNA replicative activity in normal and pathological cartilages with special emphasis on the use of flow cytometry; a continued definition of the products and rates of synthesis of normal and pathologic cartilages utilizing a variety of biochemical and metabolic techniques including the cell-free systems; a continued study of a variety of normal and pathological control mechanisms of the metabolic (both anabolic and catabolic) activities of normal and abnormal cartilages; a search for intracellular and cell membrane markers of disease states in normal and pathologic cartilages, chiefly by use of the flow cytometer; and continued attempts at isolation, identification, purification and determination of optimal conditions, substrate, cofactors and inhibitors for degradative enzymes in normal and diseased articular and epiphyseal cartilages.