NEUROCHEMISTRY OF THE DIENCEPHALON

拟议研究的目的是继续调查 下丘脑肽在调节分泌中的作用 脑下垂体前叶和控制其他身体功能。 的 这项工作的目标包括： 1.从猪下丘脑组织中分离出 催乳素释放抑制激素（PIF），催乳素释放激素 (PRH)，一种抗有丝分裂（生长抑制）因子，更大，更碱性 CRF形式和其他具有生物活性的肽。 2.这些肽的结构测定。 3.这些神经激素及其类似物的合成。 一些肽 还将合成片段以确定 CRF和GH-RF的放射免疫分析 4.生物学、化学和免疫学特性的比较 猪下丘脑激素与其他种属的下丘脑激素以及 结构差异和构效关系测定 各种下丘脑激素的关系。 5.产生针对这些肽的抗血清，用于开发 放射免疫测定和免疫细胞化学定位研究。 6.对天然下丘脑进行了广泛的生理和生化研究 激素、合成激素及其合成类似物，包括 半衰期、代谢和灭活研究。 这些研究 对于规划现实的临床试验很重要。 调查 长期服用下丘脑激素对某些 内分泌依赖性或激素敏感性肿瘤也将进行。 7.继续寻找卵泡刺激素释放激素 促性腺激素释放激素（FSH）是与十肽促性腺激素释放激素（Gn-RH）不同的促性腺激素释放激素（FSH）。 8.下丘脑肽调脂作用的研究 动员，食欲和胃酸分泌;纯化，分离， 这些肽的结构和合成的测定。 9.为临床医生提供关于自然和 用于规划人体临床试验的合成材料。

The objective of the proposed research is the continued investigation of the role the hypothalamic peptides play in the regulation of secretion of the anterior pituitary gland and in control of other bodily functions. The goals of this work include: 1. The isolation from porcine hypothalamic tissue of Prolactin-Release-Inhibiting Hormone (PIF), Prolactin Releasing Hormone (PRH), an antimitogenic (growth inhibiting) factor, larger and more basic forms of CRF and other peptides with biological activities. 2. The determination of structure of these peptides. 3. The synthesis of these neurohormones and their analogs. Some peptide fragments will be also synthesized in order to determine the specificity of our RIA's for CRF and GH-RF. 4. Comparison of biological, chemical and immunological properties of porcine hypothalamic hormones with those of other species and location of structural differences, and determination of structure-activity relationship for various hypothalamic hormones. 5. Generation of antisera to these peptides for the development of radioimmunoassays and immunocytochemical localization studies. 6. Extensive physiological and biochemical studies on natural hypothalamic hormones, synthetic hormones and their synthetic analogs, including investigation of half life, metabolism and inactivation. These studies will be important for planning realistic clinical tests. An investigation of the effects of chronic administration of hypothalamic hormones on some endocrine dependent or hormone sensitive tumors will be also carried out. 7. Continued search for a follicle stimulating hormone-releasing hormone (FSH-RH) distinct from the decapeptide LH-RH (Gn-RH). 8. The investigation of the effect of hypothalamic peptides on lipid mobilization, appetite and gastric acid secretion; purification, isolation, determination of structure and synthesis of some of these peptides. 9. Providing of detailed information and ideas to clinicians on natural and synthetic materials for the planning of clinical tests on human subjects.