LABIAL SALIVARY GLANDS-ELEMENTAL CONCENTRATIONS STUDIES

对几个组织的研究表明与囊性纤维化有关 组织功能的自主调节异常。其中包括 对胆碱能药物的敏感性增加和敏感性降低 到β-肾上腺素能药物。如果CF粘液腺也增加了 对胆碱能激动剂敏感，然后导致粘液增加 分泌物可能与观察到的支气管病理生理有关。 这些病人。然而，之前的一项研究(芒果，J Dent Res 60,797， 1981)利用人的唾液腺--腮腺--来 关于慢性肾功能异常的相反结论 自主控制。因此，重要的是要确定监管如何 人的粘液腺功能在慢性萎缩性胃炎中受到影响。 这一建议利用了对刺激引起的变化的测量 细胞内元素浓度用于检测Cf相关的变化 对副交感神经和拟交感神经药物的敏感性。元素 人唇腺细胞内不同细胞器的药物浓度 在不同实验条件下体外孵化后进行测量 采用X射线微区分析。时间和空间集中度相关性 在胆碱能和肾上腺素能激动剂刺激腺体后 使用适当的编码和计算机辅助分析进行分析 变异技术。还将使用以下工具分析空间依赖关系 数字成像技术。 体外结果将不仅允许对改变的 对胆碱能和肾上腺素能激动剂的敏感性，但也对疾病敏感 静息细胞内元素浓度的相关变化 此前报道为腮腺。此外，本研究还指出， 将扩展先前的发现，允许确定 发生浓度变化的细胞内细胞器。在……里面 此外，Co-EDTA的使用将为检测提供一种客观的方法 细胞损伤的准备程序，以及变量的使用 渗透压(定义为Na+K+C1之和)将允许估计 与疾病和刺激相关的渗透驱动力的变化。多数 重要的是，通过活检获得的人类唇腺组织的使用 患有CF的患者将最大限度地提高结果将是 与配置文件相关。

Studies in several tissues have demonstrated CF (cystic fibrosis)-related abnormalities in autonomic regulation of tissue function. Among these are an increased sensitivity to cholinergic agents and a decreased sensitivity to beta-adrenergic agents. If CF mucous glands also have increased sensitivity to cholinergic agonists, then the resulting increased mucus secretion might contribute to the bronchial pathophysiology observed in these patients. However, a previous study (Mangos, J Dent Res 60, 797, 1981) utilizing a serous human salivary gland--the parotid--came to just the opposite conclusion with regard to CF-related abnormalities in autonomic control. Hence, it is important to determine how the regulation of human mucous salivary gland function is affected in CF. This proposal utilizes measurements of stimulation induced changes in intracellular elemental concentrations to test for CF-related changes in sensitivity to parasympathomimetic and sympathomimetic agents. Elemental concentrations in different intracellular organelles in human labial glands incubated in vitro under different experimental conditions will be measured using X-ray microanalysis. Temporal and spatial concentration dependencies following gland stimulation with cholinergic and adrenergic agonists will be analyzed using appropriate coding and computer assisted analysis of variance techniques. Spatial dependencies will also be analyzed using digital imaging techniques. The in vitro results will not only allow statistical tests for altered sensitivities to cholinergic and adrenergic agonists, but also for disease related changes in resting intracellular elemental concentrations as previously reported for the parotid gland. Moreover, the present study will extend previous findings by allowing a determination of the intracellular organelles in which the concentration changes occur. In addition, use of Co-EDTA will provide an objective method for detecting cells injured in the preparatory procedure, and the use of the variable osmols (defined as the sum of Na + K + C1) will allow an estimation of disease and stimulation related changes in osmotic driving forces. Most importantly, the use of human labial gland tissues obtained by biopsy from patients with CF will maximize the likelihood that the results will be relevant to CF.