Integron and omics based acceleratation of industrial strain development

该项目旨在提供一套新的使能技术，这些技术将在速度和效率上产生一个台阶式的变化，从而可以构建工程微生物用于工业应用。这一改进将通过将重组工业生物基因组内基因的新方法与先进的代谢组学技术和直接筛选方法相结合来实现，以鉴定具有改进的工艺效率的菌株。该项目的成果将提供克服迭代应变改进的通常耗时费力的经验过程的方法。最初的应用是快速改进用于生物乙醇生产的酵母菌株，但正在使用的一套使能工具将能够加速微生物的开发，几乎可以用于任何工业应用。

This project aims to provide a new set of enabling technologies which will create a step change in the speed and efficiency with which engineered microbes can be constructed for industrial application. This improvement will be achieved by combining novel methods to recombine genes within the genome of industrial organisms with advanced metabolomics techniques and direct screening methods to identify strains with improved process efficiency. The project output will provide methods to overcome the normally time-consuming and laborious empirical process of iterative strain improvement. The initial application is to rapidly improve yeast strains used for bioethanol production but the suite of enabling tools being employed would be capable of accelerating the development of microbes for virtually any industrial application