RADIATION STUDIES IN VASCULAR AND LYMPHATIC TISSUE

体内：使用Sandison-Clark兔耳腔微血管模型 在新西兰白色雄性家兔中，将进行连续的体内观察， 在成熟的微血管和淋巴管上进行，以评估 辐射的影响。 研究了以下参数：1）血管 长度，2）血管表面积，3）血管体积，4）血管间 距离或血管密度和5）血管壁厚度作为 损伤后的剂量和时间。 辐射将是小剂量的单次剂量， 200拉德，分次剂量和超分割，并通过连续 辐射 将使用定量和定性标准进行分析 小动脉、毛细血管和小静脉的损伤和修复程度。 迟发效应是本研究的主要目的。 淋巴 血管损伤将使用以下指标量化早期和中期效应： 相同的方法和参数。 体外：比较主要 内皮细胞的培养物、亚培养物和损伤培养物，光滑的 肌肉细胞和血管成纤维细胞将进行，以提供 用于体内实验的预测信息。 或者，捐助者 在兔体内对组织进行辐照，并在体外对组织进行评分。 组织培养 将确定对细胞培养物的晚期效应， 贯彻 从这些研究中，我们打算将晚期损伤与 记录的患者的血管和淋巴晚期组织损伤 文学作品 这些研究应该为放射治疗师提供 信息导致治疗策略的发展， 最大限度地减少晚期血管和淋巴损伤， 癌症管理

IN VIVO: Using the Sandison-Clark rabbit ear chamber microvascular model in New Zealand white male rabbits sequential in vivo observations will be made on the mature microvasculature and lymphatic channels to evaluate the effects of radiation. The following parameters are studied: 1) vascular lengh, 2) vascular surface area, 3) vascular volume, 4) intervessel distance or vascular density and 5) vessel wall thickness as a function of dose and time after injury. Radiation will be small single doses, up to 200 rads, fractionated doses and hyperfractionation and by continuous radiation. Quantitative and qualitative criteria will be used to analyze the extent of injury and repair in arterioles, capillaries, and venules. Late effects are the main objective of this investigation. Lymphatic vessels injury will be quantitated for early and intermediate effects using the same methodology and parameters. IN VITRO: Studies comparing primary cultures, sub-cultures and wounded cultures of endothelial cells, smooth muscle cells and vascular fibroblasts will be carried out to provide predictive information for the in vivo experiments. Alternatively, donor tissue is irradiated in vivo in rabbits and will be scored in vitro in tissue culture. Determination of late effects on cell cultures will be carried out. From these studies we intend to correlate late injury of blood vessels and lymphatic late tissue injury of patients documented in the literature. These studies schould provide the radiotherapist with the information leading to development of treatment strategies which will minimize late vascular and lymphatic injury and decrease morbidity in cancer management.