DIAGNOSTIC RADIOLOGY IMAGING AND METABOLISM OF TUMOR

动物研究的总体目标是提供新的 体内31 P-核磁共振（NMR）相关信息 11 C或18F-2-脱氧葡萄糖（2DG）和11 C-甲硫氨酸正电子光谱 放射性断层扫描（PET）在肿瘤代谢评估中的应用 在未治疗和治疗的肿瘤生长的不同阶段， （化疗）动物。 更具体的目标包括定量肿瘤高 能量磷酸盐代谢物和pH使用31 P-NMR光谱法（两者均在 体内和体外），使用PET的葡萄糖摄取和氨基酸摄取 使用生物化学扫描、肿瘤蛋白质合成和ATP水平 技术和肿瘤形态学使用计算机轴向断层扫描（CT）， 核磁共振质子成像和组织学。 上述参数将在 两种动物肿瘤模型（兔VX-2后肢肌肉肿瘤和成人 犬脑肿瘤）在肿瘤生长的各个阶段和随后 静脉或动脉化疗。 研究结果 应该提供有关肿瘤生物学的重要信息， 使用体内成像和光谱的潜在临床价值 监测肿瘤自然病程中代谢变化的技术 生长和后续治疗。 结果的应用可以提供 一种监测癌症治疗和确定预后的新方法。

The overall goal of the proposed animal research is to provide new information relating in vivo 31P-Nuclear Magnetic Resonance (NMR) Spectroscopy to 11C or 18F-2-deoxyglucose (2DG) and 11C-Methionine Positron Emission Tomography (PET) Scanning in the evaluation of tumor metabolism during various stages of tumor growth in both untreated and treated (chemotherapy) animals. More specific aims include quantitating tumor high energy phosphate metabolites and pH using 31P-NMR Spectroscopy (both in vivo and in vitro), glucose uptake and amino acid uptake using PET Scanning, tumor protein synthesis and ATP levels using biochemical techniques and tumor morphology using computerized axial tomography (CT), NMR proton imaging and histology. The above parameters will be compared in two animal tumor models (Rabbit VX-2 hind limb muscle tumor and adult canine brain tumor) during various stages of tumor growth and following either intravenous or intraarterial chemotherapy. Results of the studies should provide important information relating to tumor biology and the potential clinical value of using in vivo imaging and spectroscopy techniques to monitor metabolic changes during the natural history of tumor growth and following therapy. The application of the results could provide a new approach to monitoring cancer therapy and determining prognosis.